DR. ROGER MAYEDA CLEMMONS, DVM, PHD
Veterinary at 16 Ave, Gainesville, FL
License number
Florida VM2707
Category
Veterinary
Type
Veterinarian
Address
Address
2015 SW 16Th Ave, Gainesville, FL 32608
Phone
(352) 328-1794
Professional information
Membrane-Strip Reagent Serodiagnostic Apparatus And Method
US Patent:
5030555, Jul 9, 1991
Filed:
Sep 12, 1988
Appl. No.:
7/243257
Inventors:
Roger M. Clemmons - Gainesville FL
Assignee:
University of Florida - Gainesville FL
International Classification:
G01N 33538, G01N 33545, G01N 33549, G01N 33569
US Classification:
435 5
Abstract:
For the serodiagnosis of a fluid sample, it is particularly advantageous to use a device comprising (A) a supported, porous membrane wherein a first immunoreagent is bound so as to be capable of binding a foreign analyte and forming a complex when the foreign analyte is brought into contact therewith; and (B) a matrix which presents a first surface and an opposing second surface and which contains a second, labeled immunoreagent that is capable of binding the foreign analyte to form a labeled complex when the foreign analyte is sandwiched between the first immunoreagent and the second immunoreagent. In such a device, the first surface of the matrix is adjacent to a surface of the membrane, (ii) the matrix is wettable by or soluble in an aqueous fluid, and (iii) the second immunoreagent is mobilized when the matrix is wetted.
Liquid-Phase Immunodiagnostic Assay
US Patent:
5637473, Jun 10, 1997
Filed:
Nov 28, 1994
Appl. No.:
8/345283
Inventors:
Roger M. Clemmons - Gainesville FL
Assignee:
University of Florida Research Foundation, Inc. - Gainesville FL
International Classification:
G01N 33536
US Classification:
435 791
Abstract:
Reagents for a liquid-phase immunodiagnostic assay (LIDA) method comprise a first enzyme; a second enzyme; a first agent which is capable of binding with an analyte to form a complex, the agent being attached to one of the first and second enzymes; and a complex-binding agent attached to the remaining enzyme, wherein the first enzyme is capable of interacting with a substrate for the first enzyme together with any necessary additional substrates for the first enzyme to produce a substrate for the second enzyme, and wherein the second enzyme is capable of interacting with the substrate produced by the first enzyme together with any necessary additional substrates, such that occurrence of the second of the interactions is detectable. The reagent optionally further comprises a scavenger substance capable of inactivating the substrate produced by the first enzyme.