Inventors:
Gary Magnant - Topsfield MA, US
Michael Finney - San Francisco CA, US
Todd Barbera - Marblehead MA, US
William Bowers - Ipswich MA, US
Diane Kozwich - Nottingham NH, US
Samuel Seymour - Marblehead MA, US
Robert Nelson - Sioux Falls SD, US
T. Boles - Bedford MA, US
Assignee:
SAGE SCIENCE, INC. - Beverly MA
International Classification:
C07K 1/26, C07K 1/28, C25B 9/08
US Classification:
204459000, 204456000, 204461000, 204464000, 204606000, 204610000, 204614000, 073864910
Abstract:
Devices and methods are provided for more efficiently performing electrophoresis and electroblotting. A sandwich structure includes an electrophoresis gel affixed to a blotting membrane. A gel casting and/or running frame is used to hold the sandwich. A method and composition that allows separation of the gel and membrane after performing a combined electrophoresis and electroblotting operation so as to allow further operations to be performed individually upon the membrane, gel or both. A uniform electrophoretic field may be created by surrounding the sandwich structure using an insulating fluid; the insulating fluid is then swapped for a conducting fluid to allow application of an electroblotting field. An apparatus automatically manages fluid exchange and actuation of electrophoresis and electroblotting electrodes. A plurality of parallel cavities may be used to hold multiple gels or gel membrane sandwiches.