ROBERT LEFKOWITZ, M.D.
Osteopathic Medicine at Erwin Rd, Durham, NC
License number
North Carolina 18793
Category
Osteopathic Medicine
Type
Cardiovascular Disease
Address
Address
2100 Erwin Rd, Durham, NC 27710
Phone
(919) 620-4467
Organization information
See more information about ROBERT LEFKOWITZ at bizstanding.comRobert Lefkowitz MD
PO Box 3821, Durham, NC 27710
Industry:
Internist
Phone:
(919) 684-2974 (Phone)
Robert Joseph Lefkowitz
Professional information
Method Of Screening The Activity Of The Smoothened Receptor To Identify Theraputic Modulation Agents Or Diagnose Disease
US Patent:
2009026, Oct 22, 2009
Filed:
Dec 14, 2006
Appl. No.:
12/097697
Inventors:
Wei Chen - Chapel Hill NC, US
Lawrence S. Barak - Durham NC, US
Marc G. Caron - Hillsborough NC, US
Robert Lefkowitz - Durham NC, US
Lawrence S. Barak - Durham NC, US
Marc G. Caron - Hillsborough NC, US
Robert Lefkowitz - Durham NC, US
International Classification:
A61K 51/00, C12Q 1/48, A61K 49/00
US Classification:
424 111, 435 15, 424 91
Abstract:
The invention relates to a screening method for the Smoothened receptor for testing compositions as potential Smoothened receptor ligands, either agonist or antagonist activity, by use of an arrestin-reporter molecule conjugate. It also relates to testing cells and individuals by administering a smoothened receptor ligand-reporter molecule conjugate and observing locations where the ligand binds and then using an increased number of surface Smoothened receptors compared to a pre-established criteria as an indication of a cancerous growth or tumor.
Robert Lefkowitz, Durham NC
Specialties:
Internist
Address:
2100 Erwin Rd, Durham, NC 27705
Education:
Columbia University, College of Physicians and Surgeons - Doctor of Medicine
Massachusetts General Hospital - Fellowship - Cardiology
Massachusetts General Hospital - Residency - Family Medicine
New York Presbyterian Hospital-Columbia Presbyterian Center - Residency - Family Medicine
Massachusetts General Hospital - Fellowship - Cardiology
Massachusetts General Hospital - Residency - Family Medicine
New York Presbyterian Hospital-Columbia Presbyterian Center - Residency - Family Medicine
Board certifications:
American Board of Internal Medicine Certification in Internal Medicine
Dr. Robert Lefkowitz, Durham NC - MD (Doctor of Medicine)
Specialties:
Internal Medicine
Address:
3821 Duke Univ Medical Ctr, Durham 27710
2100 Erwin Rd, Durham 27705
2100 Erwin Rd, Durham 27705
(919) 620-4467 (Phone)
2100 Erwin Rd, Durham 27705
2100 Erwin Rd, Durham 27705
(919) 620-4467 (Phone)
Certifications:
Internal Medicine, 1972
Awards:
Healthgrades Honor Roll
Languages:
English
Education:
Medical School
Columbia Univ Coll Of Physicians and Surgeons
Graduated: 1966
Colum P&S
Mass Genl Hosp-Harvard
Columbia Univ Coll Of Physicians and Surgeons
Graduated: 1966
Colum P&S
Mass Genl Hosp-Harvard
Inhibition Of Arrestin Mediated Effects Prolongs And Potentiates Opioid Receptor-Mediated Analgesia
US Patent:
6528271, Mar 4, 2003
Filed:
Dec 22, 1999
Appl. No.:
09/469554
Inventors:
Laura M. Bohn - Durham NC
Marc G. Caron - Hillsborough NC
Robert J. Lefkowitz - Durham NC
Marc G. Caron - Hillsborough NC
Robert J. Lefkowitz - Durham NC
Assignee:
Duke University - Durham NC
International Classification:
C07K 1400
US Classification:
435 72, 435 4, 530350
Abstract:
The present invention provides a arrestin knockout mouse useful for screeening compounds for efficacy in controlling pain, methods of controlling pain in subjects by inhibiting binding of arrestin to phosphorylated opioid receptors, and methods of screening a compound for activity in potentiating opioid receptor agonist activity (e. g. , morphine activity) by determining whether or not said compound inhibits arrestin binding to a phosphorylated opioid receptor.
Expression Of G Protein Coupled Receptors In Yeast
US Patent:
6168927, Jan 2, 2001
Filed:
Apr 8, 1998
Appl. No.:
9/056920
Inventors:
Klim King - Durham NC
Henrik G. Dohlman - Berkeley CA
Marc G. Caron - Durham NC
Robert J. Lefkowitz - Durham NC
Henrik G. Dohlman - Berkeley CA
Marc G. Caron - Durham NC
Robert J. Lefkowitz - Durham NC
Assignee:
Duke University - Durham NC
International Classification:
G01N 3353
US Classification:
435 72
Abstract:
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein. alpha. subunit (mammalian G. sub. alpha. ). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein. alpha. -subunit (yeast G. sub. alpha. ) The cells are useful for screening compounds which affect the rate of dissociation of G. sub. alpha. from G. sub. beta. gamma. in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
Methods Of Testing In Yeast Cells For Agonists And Antagonists Of Mammal G-Protein Coupled Receptors
US Patent:
5482835, Jan 9, 1996
Filed:
Jun 3, 1993
Appl. No.:
8/071355
Inventors:
Klim King - Durham NC
Henrik G. Dohlman - Berkeley CA
Marc G. Caron - Durham NC
Robert J. Lefkowitz - Durham NC
Henrik G. Dohlman - Berkeley CA
Marc G. Caron - Durham NC
Robert J. Lefkowitz - Durham NC
Assignee:
Duke University - Durham NC
International Classification:
C12N 116, C12N 1511, C12N 1512, C12Q 102, C12Q 168, G01N 33566
US Classification:
435 6
Abstract:
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein. alpha. subunit (mammalian G. sub. alpha. ). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein. alpha. -subunit (yeast G. sub. alpha. ). The cells are useful for screening compounds which affect the rate of dissociation of G. sub. alpha. from G. sub. beta. gamma. in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
Methods Of Testing For Agonists And Antagonists Of Cell Surface Receptors
US Patent:
2003021, Nov 20, 2003
Filed:
Jan 17, 2003
Appl. No.:
10/347141
Inventors:
Klim King - Durham NC, US
Henrik Dohlman - Berkeley CA, US
Marc Caron - Durham NC, US
Robert Lefkowitz - Durham NC, US
Henrik Dohlman - Berkeley CA, US
Marc Caron - Durham NC, US
Robert Lefkowitz - Durham NC, US
Assignee:
Duke University
International Classification:
G01N033/53, G01N033/567, C12N001/18
US Classification:
435/007200, 435/254200
Abstract:
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein a subunit (mammalian G). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein -subunit (yeast G). The cells are useful for screening compounds which affect the rate of dissociation of G from G in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.
Radiolabled Cyclopamine Assay For The Smoothened Receptor
US Patent:
2011023, Sep 29, 2011
Filed:
Jun 16, 2009
Appl. No.:
12/999922
Inventors:
Wei Chen - Chapel Hill NC, US
Lawrence S. Barak - Durham NC, US
Jiangbo Wang - Durham NC, US
Robert J. Lefkowitz - Durham NC, US
Herbert Kim Lyerly - Chapel Hill NC, US
Lawrence S. Barak - Durham NC, US
Jiangbo Wang - Durham NC, US
Robert J. Lefkowitz - Durham NC, US
Herbert Kim Lyerly - Chapel Hill NC, US
International Classification:
G01N 23/00
US Classification:
435 72
Abstract:
The present invention discloses novel methods for screening compositions for both agonist and antagonist activity on the smoothened receptor. The assay tests ligands on the activity of the receptor using a cyclopamine binding and ultra high receptor expression.
Preventing Desensitization Of Receptors
US Patent:
2006010, May 11, 2006
Filed:
Nov 15, 2005
Appl. No.:
11/274047
Inventors:
Jonathan Stamler - Chapel Hill NC, US
Robert Lefkowitz - Durham NC, US
Erin Whalen - Durham NC, US
Walter Koch - Durham NC, US
Claude Plantadosi - Durham NC, US
Robert Lefkowitz - Durham NC, US
Erin Whalen - Durham NC, US
Walter Koch - Durham NC, US
Claude Plantadosi - Durham NC, US
International Classification:
A61K 38/05
US Classification:
514018000
Abstract:
Desensitization of receptors that control disease is prevented by inhibiting G-protein receptor kinases. This has applicability, e.g., for patients with heart failure or on a left ventricular heart device or a heart pump after surgery or about to undergo surgery and at high risk for a cardiac event or on an opiate or addicted to opiate or with cystic fibrosis or rheumatoid arthritis.
Inhibition Of Betaarrestin Mediated Effects Prolongs And Potentiates Opioid Receptor-Mediated Analgesia
US Patent:
2003009, May 22, 2003
Filed:
Jan 3, 2003
Appl. No.:
10/336276
Inventors:
Laura Bohn - Durham NC, US
Marc Caron - Hillsborough NC, US
Robert Lefkowitz - Durham NC, US
Marc Caron - Hillsborough NC, US
Robert Lefkowitz - Durham NC, US
International Classification:
A01K067/027, G01N033/53, A61K031/485
US Classification:
800/018000, 514/282000, 435/007100
Abstract:
The present invention provides a arrestin knockout mouse useful for screening compounds for efficacy in controlling pain, methods of controlling pain in subjects by inhibiting binding of arrestin to phosphorylated opioid receptors, and methods of screening a compound for activity in potentiating opioid receptor agonist activity (e.g., morphine activity) by determining whether or not said compound inhibits arrestin binding to a phosphorylated opioid receptor.