DR. ROBERT H SINGER, DPM
Podiatry at 45 St, New York, NY

6534266, Mar 18, 2003

Apr 21, 2000

09/556134

Robert H. Singer - New York NY

Albert Einstein College of Medicine of Yeshiva University - Bronx NY

C12Q 168

435 6, 435 72, 435 721, 435174, 536 231, 536 243, 536 2431, 536 266

An in situ hybridization method for detecting and specifically identifying transcription of a multiplicity of different target sequences in a cell is disclosed. The method includes assigning a different bar code to at least five target sequences, with each target sequence containing at least one predetermined subsequence. Each bar code contains at least one fluorochrome, and at least one bar code comprises at least two different, spectrally distinguishable fluorochromes. A probe set specific for each target sequence is provided in the method. Each probe set contains a hybridization probe complementary to each subsequence in the target sequence. Each probe is labeled with a fluorochrome, and the fluorochromes in each probe set collectively correspond to the bar code for the target sequence of that probe set.

6586240, Jul 1, 2003

Sep 6, 2000

09/656010

Robert H. Singer - New York NY
Edouard Bertrand - Montpelier, FR

Albert Einstein College of Medicine of Yeshiva University - Bronx NY

C12N 1585

435325, 435 6, 4353201, 435440, 4352523, 435 8, 536 231, 536 234

A method for visualizing the location and movement of a specific RNA of interest in a living cell, in real time, is disclosed. The method includes the following steps: (a) providing a DNA encoding the RNA, which RNA includes a protein-binding site; (b) providing a nucleic acid encoding a fusion protein, which fusion protein comprises a fluorescent domain and an RNA-binding domain; (c) introducing the DNA encoding the RNA, and the nucleic acid encoding the fusion protein, into a eukaryotic cell so that the DNA encoding the RNA and the nucleic acid encoding the fusion protein are expressed in the cell; and (d) detecting fluorescence outside the nucleus or inside the nucleus of the cell, with the fluorescence being from the fusion protein bound to the RNA. In some embodiments, the fusion protein also includes an intracellular localization domain.

6203986, Mar 20, 2001

Oct 22, 1998

9/177268

Robert H. Singer - New York NY
Edouard Bertrand - 34000 Montpellier, FR

A61B 1000, C12Q 168, C12N 1540

435 6

A method for visualizing the location and movement of a specific RNA of interest in a living cell, in real time, is disclosed. The method includes the following steps: (a) providing a DNA encoding the RNA, which RNA includes a protein-binding site; (b) providing a nucleic acid encoding a fusion protein, which fusion protein comprises a fluorescent domain and an RNA-binding domain; (c) introducing the DNA encoding the RNA, and the nucleic acid encoding the fusion protein, into a eukaryotic cell so that the DNA encoding the RNA and the nucleic acid encoding the fusion protein are expressed in the cell; and (d) detecting fluorescence outside the nucleus or inside the nucleus of the cell, with the fluorescence being from the fusion protein bound to the RNA. In some embodiments, the fusion protein also includes an intracellular localization domain.

2011031, Dec 29, 2011

May 7, 2009

12/736885

Robert H. Singer - New York NY, US
Rosanna Pezo - Bronx NY, US
Leonard H. Augenlicht - Redding CT, US

C12Q 1/68

435 611

The present invention generally relates to methods for determining tumor resistance or sensitivity to chemotherapeutic agents and the likelihood of tumor reoccurrence based on the expression levels of genes known to correlate to the chemotherapeutic agent. In particular, the expression levels of TYMS, MRGX, ATP7B and/or BAK in tumor cells, as measured by the number of active transcription sites detected by fluorescence in situ hybridization (FISH), are predictive of resistance and sensitivity to chemotherapy and the likelihood of reoccurrence following chemotherapy treatment.