Inventors:
Diana McWilliams - Bonne Terre MO, US
Robert Becker - Wildwood MO, US
Charles Bolten - Kirkwood MO, US
Pamela De Ciechi - O'Fallon MO, US
Shawn Miller - Fenton MO, US
Andrew Pinz - O'Fallon MO, US
Jeffrey Surry - Florissant MO, US
International Classification:
C12Q001/68, G06F019/00, G01N033/48, G01N033/50
Abstract:
A high throughput RNA laboratory protocol is provided for the extraction and maintenance of a sufficient quantity of high quality RNA during sample preparation in order to analyze several genes at a time with assistance of computer analysis. The subject invention includes a method for analyzing RNA comprising the steps of extracting RNA from a complex biological construct in sufficient quantities to provide accurate RNA data, transferring RNA to an apparatus that maintains the RNA and necessary reagents at a temperature of between about 0 to 10° C., and analyzing RNA with a computer generated mathematical analysis of the data to access the presence of RNA and ultimately test the efficacy of a drug.