Inventors:
Herbert A. Marble - Boulder CO
Robert H. Davis - Boulder CO
Assignee:
Regents of the University of Colorado, The - Boulder CO
International Classification:
C12P 1934
Abstract:
The instant invention provides a method for producing RNA enzymatically in a semi-batch or continuous-flow bioreactor using a DNA template immobilized to a solid support through a linkage having a single-stranded overhang extending from a noncoding strand. The coding strand remains dissociable from the immobilized noncoding strand. The immobilized RNA template is reused in at least sixteen rounds of transcription reaction. The coding strand can be replaced with a fresh DNA strand encoding the same or a different RNA.