Robert Charles Davis
Engineers at 4 St, Boulder, CO

License number
Colorado 7881
Issued Date
Oct 7, 1967
Renew Date
Sep 1, 2005
Expiration Date
Aug 31, 2007
Type
Professional Engineer
Address
Address
2299 4Th St, Boulder, CO 80302

Professional information

Robert Davis Photo 1

Strategy For The Production Of Rna From Immobilized Templates

US Patent:
5700667, Dec 23, 1997
Filed:
May 18, 1995
Appl. No.:
8/443958
Inventors:
Herbert A. Marble - Boulder CO
Robert H. Davis - Boulder CO
Assignee:
Regents of the University of Colorado, The - Boulder CO
International Classification:
C12P 1934
US Classification:
435 913
Abstract:
The instant invention provides a method for producing RNA enzymatically in a semi-batch or continuous-flow bioreactor using a DNA template immobilized to a solid support through a linkage having a single-stranded overhang extending from a noncoding strand. The coding strand remains dissociable from the immobilized noncoding strand. The immobilized RNA template is reused in at least sixteen rounds of transcription reaction. The coding strand can be replaced with a fresh DNA strand encoding the same or a different RNA.