ROBERT CHARLES ALLEN, D.O.
Osteopathic Medicine at Catlin Ct, San Antonio, TX

5451402, Sep 19, 1995

Nov 22, 1994

8/343781

Robert C. Allen - San Antonio TX

ExOxEmis, Inc. - Little Rock AR

A61K 3844, A61K 31195, A61L 200, C12N 908, C12N 902, C12N 904

424 944

Methods and compositions are provided for killing or inhibiting the growth of yeast or sporular microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or bromide, with a haloperoxidase and at least one antimicrobial activity enhancing agent. Suitable antimicrobial activity enhancing agents include certain. alpha. -amino acids, and are preferably compounds of the formula: ##STR1## wherein R. sub. 1 is hydrogen, an unsubstituted, or hydroxy or amino substituted, straight or branched chain alkyl group having from 1 to 6 carbon atoms, or an unsubstituted, or hydroxy or amino substituted arylalky group having from 7 to 12 carbon atoms, and R. sub. 2 is hydrogen or a straight or branched chain alkyl group having from 1 to 6 carbon atoms. The significantly enhanced haloperoxidase antiyeast and antispore activities make the methods and compositions of the invention highly useful in the therapeutic or prophylactic antiseptic treatment of human or animal subjects and in in vitro applications for disinfection or sterilization of vegetative microbes, yeasts, and bacterial and fungal spores. Suitable haloperoxidases for use in the methods and compositions of the invention include eosinophil peroxidase (EPO) and myeloperoxidase (MPO).

5389369, Feb 14, 1995

Aug 2, 1993

8/100780

Robert C. Allen - San Antonio TX

ExOxEmis, Inc. - Little Rock AR

C12N 908, C12N 902, C12N 904, A61K 3750, A61K 31195, A61L 200

424 944

Methods and compositions are provided for killing or inhibiting the growth of yeast or sporular microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or bromide, with a haloperoxidase and at least one antimicrobial activity enhancing agent. Suitable antimicrobial activity enhancing agents include certain. alpha. -amino acids, and are preferably compounds of the formula: ##STR1## wherein R. sub. 1 is hydrogen, an unsubstituted, or hydroxy or amino substituted, straight or branched chain alkyl group having from 1 to 6 carbon atoms, or an unsubstituted, or hydroxy or amino substituted arylalky group having from 7 to 12 carbon atoms, and R. sub. 2 is hydrogen or a straight or branched chain alkyl group having from 1 to 6 carbon atoms. The significantly enhanced haloperoxidase antiyeast and antispore activities make the methods and compositions of the invention highly useful in the therapeutic or prophylactic antiseptic treatment of human or animal subjects and in in vitro applications for disinfection or sterilization of vegetative microbes, yeasts, and bacterial and fungal spores. Suitable haloperoxidases for use in the methods and compositions of the invention include eosinophil peroxidase (EPO) and myeloperoxidase (MPO).

5565197, Oct 15, 1996

Jan 12, 1995

8/371585

Robert C. Allen - San Antonio TX

ExOxEmis, Inc. - Little Rock AR

A61K 3843, A61K 3844, C12N 902, C12N 908

424 944

Methods and compositions for the prophylaxis and/or treatment of sexually transmitted diseases (STDs) are disclosed, in which amounts of a haloperoxidase, such as myeloperoxidase or eosinophil peroxidase, and a semen substrate-specific oxidase are administered to a human or animal subject in the environment of sexually transmitted fluids. In the presence of semen, the semen substrate-specific oxidase catalyzes the production of hydrogen peroxide, which in turn is utilized by the haloperoxidase to selectively inhibit pathogenic microbes present in the sexually transmitted fluids. At high concentration levels, the compositions additionally exhibit spermicidal properties.

5756090, May 26, 1998

Jun 1, 1995

8/457524

Robert C. Allen - San Antonio TX

EOE, Inc. - Little Rock AR

C12N 908, A61K 3750, A61K 31195

424 944

Methods and compositions are disclosed for producing air-activated, i. e. , oxygen (O. sub. 2) activated, disinfectant-sterilent solutions. Solutions containing a haloperoxidase (i. e. , a halide:hydrogen peroxide (H. sub. 2 O. sub. 2) oxidoreductase, such as myeloperoxidase, eosinophil peroxidase or lactoperoxidase) plus a halide or combination of halides (i. e. , chloride, bromide and/or iodide), an oxidase (i. e. , a substrate: O. sub. 2 oxidoreductase) capable of generating H. sub. 2 O. sub. 2, and a substrate specific for that oxidase, are separately prepared under aerobic conditions, but all of the component solutions are made anaerobic prior to final combination and mixing. The anaerobic formulations are dispensed into containers capable of maintaining the anaerobic condition (e. g. , pressurized canisters). Dispensing the solution at the time of use exposes the formulation to air (i. e. , O. sub.

6294168, Sep 25, 2001

Jul 7, 1994

8/271583

Robert Charles Allen - San Antonio TX

ExOxEmis, Inc. - Little Rock AR

A61K 3844, C12N 908

424 944

Haloperoxidases are used to selectively bind to and, in the presence of peroxide and halide, inhibit the growth of target microbes without eliminating desirable microbes or significantly damaging other components, such as host cells, in the environment of the target microbe. When a target microbe, e. g. , a pathogenic microbe, has a binding capacity for haloperoxidase greater than that of a desired microbe, e. g. , members of the normal flora, the target microbe selectively binds the haloperoxidase with little or no binding of the haloperoxidase by the desired microbe. In the presence of peroxide and halide, the target bound haloperoxidase catalyzes halide oxidation and facilitates the disproportionation of peroxide to singlet molecular oxygen at the surface of the target microbe. The lifetime of singlet molecular oxygen restricts damage to the surface resulting in selective killing of the target microbe with a minimum of collateral damage to the desired microbe or physiological medium. Methods and compositions provided are highly useful in the therapeutic or prophylactic antiseptic treatment of human or animal subjects, since their use can be designed to be highly effective in combatting bacterial or fungal infections without significant damage to normal flora or host cells.