DR. ROBERT CAMPBELL MCINTYRE, M.D.
Marriage and Family Therapists at Madison St, Seattle, WA

5084396, Jan 28, 1992

Sep 7, 1990

7/579627

A. Charles Morgan - Edmonds WA
Clive S. Woodhouse - Seattle WA
Robert F. McIntyre - Seattle WA

Neorx Corporation - Seattle WA

G01N 33563, A61K 3900

436513

A method for enhancing production of antibodies through immunization with insolubilized immune complexes is disclosed. Purified antigen or heterogeneous antigen mixtures may be combined with polyclonal or monoclonal antibody and the resultant complex bound to an insolubilized matrix to form insolubilized immune complexes. Methods for improving the immunogenicity of a soluble antigen and for producing monoclonal anti-iodiotypic antibodies are also disclosed. Monoclonal antibodies that are specific for a distinct, as yet unrecognized epitope may be produced by another disclosed method. Insolubilized immune complexes, comprising antigen and antibody that is ether directly linked to Sepharose. RTM. or absorbed onto insolubilized protein A, and immunosorbents, comprising antibody absorbed onto insolubilized protein A, are also disclosed.

4879225, Nov 7, 1989

Mar 11, 1987

7/024632

Alton C. Morgan - Edmonds WA
Clive S. Woodhouse - Seattle WA
Robert F. McIntyre - Seattle WA

NeoRx Corporation - Seattle WA

C12P 2100, C12N 500, C12N 1500, A61K 3900

435 68

A method for enhancing production of antibodies through immunization with insolubilized immune complexes is disclosed. Purified antigen or heterogeneous antigen mixtures may be combined with polyclonal or monoclonal antibody and the resultant complex bound to insolubilized protein A to form insolubilized immune complexes. Methods for improving the immunogenicity of a soluble antigen and for producing monoclonal anti-idiotypic antibodies are also disclosed. Monoclonal antibodies that are specific for a distinct, as yet unrecognized epitope may be produced by another disclosed method. Insolubilized immune complexes, comprising antigen and antibody that is either directly linked to Sepharose. RTM. or absorbed onto insolubolized protein A, and immunosorbents, comprising antibody absorbed onto insolubilized protein A, are also disclosed.

4822734, Apr 18, 1989

Sep 6, 1985

6/773340

Alton C. Morgan - Edmonds WA
Robert McIntyre - Seattle WA
Clive S. Woodhouse - Edmonds WA
Paul G. Abrams - Seattle WA

NeoRx Corporation - Seattle WA

C12P 2100, C12N 1500, C12N 500, A61K 3900

435 68

Methods are disclosed for improving the efficiency of elicitation of monoclonal antibodies to glycoprotein antigens and tumor-associated antigens, and for inducing the production of IgG class monoclonal antibodies, in particular the IgG. sub. 3 subclass. The methods involve the use of a lectin/extract immunogen to stimulate the production of the desired monoclonal antibodies.

5099005, Mar 24, 1992

Sep 28, 1990

7/589984

Everett J. Nichols - Edmonds WA
Robert F. McIntyre - Seattle WA

NeoRx Corporation - Seattle WA

C07K 308

5303881

Methods of enhancing immunoglobulin fragment yield of immunoglobulins exhibiting anomalous bands when analyzed by SDS-PAGE techniques are provided. Such yield enhancement methods include the steps of desialylating the immunoglobulin; and fragmenting the immunoglobulin produced in the desialylating step. A particular advantage of the present invention is that the fragmention step can be carried out in the presence of cysteine without decreasing the quality of the immunoglobulin fragment product. Methods of enhancing the molecular weight homogeneity of immunoglobulin or fragmented immunoglobulin exhibiting anomalous bands when analyzed by SDS-PAGE techniques are also discussed. These methods include the steps of desialylating the immunoglobulin or fragmented immunoglobulin and purifying the desialylated product.