Inventors:
Eddie H. Massey - Indianapolis IN
Richard L. Jackson - Indianapolis IN
Assignee:
Eli Lilly and Company - Indianapolis IN
International Classification:
C07K 742, C07K 740
Abstract:
This disclosure provides a crystalline human proinsulin, the preparation of which comprises
(a) preparing an aqueous mixture containing from about 5 to about 50 mg/ml human proinsulin, from about 0. 1 to about 5 mg/ml of a phenolic substance, from about 0. 03 to about 0. 6 milliequivalents/ml of a salt of a cation selected from the group consisting of lithium, calcium, sodium, potassium, ammonium, magnesium, and barium, and from about 0. 2 to about 5 milliequivalents of Zn. sup. +2 cation;
(b) adjusting the aqueous mixture to a pH in the range of from about 5. 4 to about 6. 5;
(c) allowing crystal formation to occur; and
(d) recovering crystalline human proinsulin from the aqueous mixture.