Inventors:
Neal F. Bellet - Walnut Creek CA, US
Jeffrey Shindelman - Castro Valley CA, US
Thomas E. Worthy - Walnut Creek CA, US
Kimberly Haley - Belmont CA, US
Richard K-T Lee - Fremont CA, US
Jane Eunhee Kyung - Sunnyvale CA, US
Suyue Qian - Fremont CA, US
Assignee:
Cholestech Corporation - Hayward CA
International Classification:
C12M 1/34
US Classification:
4352872, 422 56, 422 57, 422 58, 4352877, 4352879, 435805, 435810, 435970, 436514, 436518, 436810
Abstract:
An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product, and a detection zone located downstream at which the detectable product can be observed. By controlling movement of the support to its first and second positions, the cassette can effectively handle a variety of samples having different flow rates.