Inventors:
Richard J. Jones - Bel Air MD
O. Michael Colvin - Baltimore MD
John Hilton - Parkton MD
Saul Sharkis - Towson MD
Assignee:
Johns Hopkins University School of Medicine - Baltimore MD
International Classification:
C12Q 132
Abstract:
Production of a stem cell enriched cell composition using essentially non-toxic methodology comprising contacting a cell mixture with a cell-permeable, fluorescent non-polar compound that reacts upon contact with intracellular enzyme aldehyde dehydrogenase or phosphokinase found substantially exclusively in stem cells so as to form a biocompatible fluorescent product that is polar and non-permeable to the membrane of the stem cells, and isolating the cells containing the marker by fluorescent cell sorting.