Inventors:
Rebeca Geffin - North Miami Beach FL, US
Richard Myers - Miami Beach FL, US
Assignee:
University Of Miami - Miami Beach FL
International Classification:
A61K 39/12, C12N 15/74, C12N 7/01, C40B 40/02, A61P 31/12
US Classification:
4242051, 435477, 4352351, 506 14
Abstract:
The invention uses recombinant technology to create infectious molecular clones that capture the sequence diversity of viral genes found in natural populations of mixed genotype viruses, such as arises during HIV infections and many other viral diseases. The invention captures the sequence diversity of different genes in these “quasi-species” populations by recombining them into in a constant genetic “backbone” for each viral species by backcrossing PCR products derived from quasispecies gene variants into this backbone in an BAC plasmid.