Inventors:
Richard C. Egen - Cheyney PA, US
Lori Ann Fortin - Bethlehem PA, US
Willie Wei Qiang Sun - Easton PA, US
International Classification:
C12P 19/04, C12N 1/20
US Classification:
435101, 435 711, 4352523, 536123
Abstract:
Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of serotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.