Richard A Myers
Pharmacy in Salt Lake City, UT

License number

Utah 103970-8912

Issued Date

Oct 28, 1992

Expiration Date

May 31, 1997

Professional information

Method Of Obtaining Small Conformationally Rigid Conopeptides

US Patent:

5885780, Mar 23, 1999

Filed:

Jul 19, 1991

Appl. No.:

7/733095

Inventors:

Baldomero M. Olivera - Salt Lake City UT
David R. Hillyard - Holiday UT
Richard A. Myers - Salt Lake City UT
Jamie K. Scott - Columbia MO
George P. Smith - Columbia MO

Assignee:

University of Utah - Salt Lake City UT
The Curators of the University of Missouri - Columbia MO

International Classification:

G01N 3353

US Classification:

435 71

Abstract:

A method for separating, identifying and purifying small, conotoxin-like rigidly conformed peptides ("conopeptides") containing multiple Cys residues comprises forming a conoeffector library, each member of which has a nucleic acid encoding a potential conopeptide sequence. The conoeffectors are expressed such that they are exposed on the surface of a bacteriophage. These bacteriophage are screened for binding to a target protein molecule, and receptors in particular, to separate and bind phage having affinity for the target protein. Reiterative screening, if required, is used to enrich and yield a phage carrying the bound conopeptide of the desired specificity and affinity. The enriched phage are then subjected to DNA sequencing to determine the conopeptide sequence including the position of the Cys residues. The chemical structure information gathered, coupled with the binding specificities to the target protein, permits the genetic or synthetic preparation of a large variety of small rigidly conformed disulfide rich peptides as pharmaceutical, pesticidal or other bioactive candidates.