Inventors:
Giacomo Vacca - Santa Clara CA, US
Richard G. Kendall - Miami FL, US
Norman R. Goldblatt - Los Altos CA, US
Michael W. Yee - Mount Shasta CA, US
Mahesh R. Junnarkar - San Jose CA, US
Assignee:
Abbott Laboratories - Abbott Park IL
International Classification:
G01N 21/00
Abstract:
A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, and for further reducing the complexity of such a cytometer or analyzer, by utilizing the technique of laser rastering in combination with a lysis-free single-dilution method. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. A lysis-free single-dilution method involves performing all the flow cytometer measurements on a sample using a single aliquot, a single lysis-free reagent solution, a single dilution, and a single pass of said dilution through the measurement apparatus. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flowcell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.