Inventors:
Adi F. Gazdar - Dallas TX
Renato V. La Rocca - Louisville KY
Cy A. Stein - New York NY
Charles E. Myers - Rockville MD
Herbert K. Oie - Rockville MD
Assignee:
The United States of America as represented by the Department of Health
and Human Services - Washington DC
International Classification:
C12N 500, C12P 2104, C12P 3300, C12P 100
Abstract:
Continuous cell lines have been established from adrenocortical corcinomas. The cell lines are maintained in fully defined serum-free, steroid-free mediums. The cells of the invention, as exemplified by NCI-H295 cells, express all of the major pathways of steroid-ogenesis, including formation of corticosteroids, mineralocorticoids and androgens.