Inventors:
WenYong Chen - Temple City CA, US
Ravi Bhatia - Duarte CA, US
Leila Su - Diamond Bar CA, US
International Classification:
C12Q 3/00, C12N 5/0787, C12N 5/09
US Classification:
435 3, 435325, 702 19, 702 27, 703 11
Abstract:
A method of generating a chronic myelogenic leukemia (CML) acquired chemoresistant culture model is provided. Such a method may comprise providing a naïve blast crisis CML cell line; administering/contacting the cell line with a mutation-inducing dose of imatinib; maintaining a culture of the treated cell line for a period of time until the treated cell line relapses and repopulates the culture; and determining the repopulated cell culture is a CML acquired chemoresistant cell line by detecting a BCR-ABL mutation, wherein the acquired chemoresistance is achieved by a BCR-ABL mutation.