RAMON LLULL
Medical Practice in Pittsburgh, PA

6316247, Nov 13, 2001

Jun 15, 1999

9/333532

Adam J. Katz - Pittsburgh PA
Ramon Llull - Pittsburgh PA
J. William Futrell - Pittsburgh PA
Marc H. Hedrick - Encino CA
Frank R. Walters - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA

C07G 1700

435267

A device and method for producing refined adipose tissue for use in autologous adipose transplantation or research wherein a sterile environment is maintained. More particularly, a system for refining tissue comprising a first flexible container, a second flexible container having a plurality of pores providing for fluid communication between the interior of the first flexible container and the interior of the first enveloping flexible container, a first port that provides communication between the exterior environment and the interior of the first flexible container and thereby provides a means for introducing fluids into the interior of the first flexible container and allowing waste effluent to exit the first container, a second flexible container, a second port for inserting tissue into the second container and expressing refined tissue out of the container, and a means for controlling the opening and closing of the first and second ports. The method comprises introducing a tissue sample into the system and subjecting it to a washing phase wherein the tissue is agitated, the waste effluent is allowed to exit the system through the first port, and the refined tissue is expressed out the second port for further refinement or transplantation.

5786207, Jul 28, 1998

May 28, 1997

8/864444

Adam J. Katz - Pittsburgh PA
Ramon Llull - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA

C07G 1700

435267

A tissue dissociation method and system for washing and dissociating tissue and producing a cellular suspension for use in autologous, cell-based therapies or research wherein a sterile environment is maintained. More particularly, a system for dissociating tissue comprising a housing unit, a first compartment having a plurality of pores providing for fluid communication between the interior of the first compartment and the interior of the housing unit, a first inlet port that provides communication between the exterior environment and the interior of the first compartment and thereby provides a means for introducing solutions into the interior of the first compartment, a second compartment, a second inlet port for introducing a tissue sample into the system, a means for agitating or rotating the second compartment in the housing unit, a means for controlling the exit of effluent from the housing unit, a first receptacle, and a centrifuge-ready second receptacle for collecting a resulting cell suspension. The method comprises introducing a tissue sample into the system and subjecting it to a washing phase and a dissociating phase wherein the tissue is agitated or rotated. The tissue may also be subjected to a sedentary phase and a phase in which a solution is added to the resulting cell suspension to cease tissue dissociation.