RALPH L BRINSTER
Veterinary in Gladwyne, PA
License number
Pennsylvania BV002937L
Category
Veterinary Medicine
Type
Veterinarian
Address
Address
Gladwyne, PA 19035
Professional information
Synthesis Of Functional Human Hemoglobin And Other Proteins In Erythroid Tissues Of Transgenic Non-Human Mammals
US Patent:
6613957, Sep 2, 2003
Filed:
Sep 1, 1999
Appl. No.:
09/388401
Inventors:
Tim M. Townes - Birmingham AL
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Assignee:
The UAB Research Foundation - Birmingham AL
International Classification:
A01K 67027
US Classification:
800 14, 800 21, 800 22, 800 25, 800 4, 800 5
Abstract:
The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the -globin locus are sufficient to result in high level expression of human - or -globin transgenes.
Nucleic Acid Vectors Comprising Dnase I Hypersensitive Sites
US Patent:
6022736, Feb 8, 2000
Filed:
Jun 6, 1995
Appl. No.:
8/471483
Inventors:
Tim M. Townes - Birmingham AL
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Assignee:
The UAB Research Foundation - Birmingham AL
The Trustees of the University of Pennsylvania - Philadelphia PA
Board of Regents of the University of Washington - Seattle WA
The Trustees of the University of Pennsylvania - Philadelphia PA
Board of Regents of the University of Washington - Seattle WA
International Classification:
C12N 1563, C12N 1509, C12N 1500, C12N 500
US Classification:
4353201
Abstract:
The present invention provides novel recombinant nucleic acid vectors which may be used to produce. alpha. -globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The present invention also provides for the transgenic animals which contain these recombinant nucleic acid vectors. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the. beta. -globin locus together with a gene of interest. According to various embodiments of the invention, the vectors may be used to create transgenic animals or to transfect cells in culture. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human. alpha. -globin gene is used to create transgenic animals which produce human. alpha. -globin protein in erythroid tissues, including red blood cells.
Method Of Obtaining Gene Product Through The Generation Of Transgenic Animals
US Patent:
4870009, Sep 26, 1989
Filed:
Dec 15, 1983
Appl. No.:
6/561644
Inventors:
Ronald M. Evans - La Jolla CA
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Assignee:
The Salk Institute for Biological Studies - San Diego CA
International Classification:
C12P 2102, C12N 1500, C12N 500, C12N 100
US Classification:
435 70
Abstract:
Mammalian genes that encode hormones are cloned and linked to strong promoter DNA sequences. The linked sequences are inserted in plasmids for amplification in prokaryotic cells, and multiple copies of the linked sequences are excised therefrom. Linked sequences are subsequently microinjected into fertilized eggs and the fertilized eggs are implanted into pseudo-pregnent females of the same species. As a result, transgenic animals are born having the linked sequences incorporated into their genomes and expressing the gene-encoded hormone. Because multiple copies of the linked sequences are frequently inserted and because production of the hormone is not limited to certain organs, as is the case with most endogenous hormones, the transgenic animals produce substantial amounts of the hormone. Hormone can be harvested from the living animal (and from its hormone-producing progeny) by extracting fluid, such as blood serum or ascites fluid, on a regular basis.
Method Of Preparing Recombinant Proteins In Transgenic Animals Containing Metallothionein Gene Elements That Bestow Tissue-Independent Copy Number-Dependent, Position-Indepedent Gene Expression
US Patent:
5583009, Dec 10, 1996
Filed:
Dec 8, 1992
Appl. No.:
7/986963
Inventors:
Richard Palmiter - Seattle WA
Eric P. Sandgren - Philadelphia PA
Ralph L. Brinster - Gladwyne PA
Eric P. Sandgren - Philadelphia PA
Ralph L. Brinster - Gladwyne PA
Assignee:
University of Washington - Seattle WA
The Trustees of the University of Pennsylvania - Philadelphia PA
The Trustees of the University of Pennsylvania - Philadelphia PA
International Classification:
C12P 2102, C12P 2100, C12N 1509, C07H 2104
US Classification:
435691
Abstract:
Vertebrate gene locus control regions linked to a structural gene of interest and inserted into a transgenic animal are capable of bestowing tissue-independent, copy number-dependent, position-independent gene expression. Recombinant proteins of interest encoded by structural genes linked to the metallothionein locus control regions may be prepared following expression in transgenic animal hosts.
Synthesis Of Functional Human Hemoglobin And Other Proteins In Erythroid Tissues Of Transgenic Animals
US Patent:
5766884, Jun 16, 1998
Filed:
Jun 6, 1995
Appl. No.:
8/466443
Inventors:
Tim M. Townes - Birmingham AL
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Assignee:
The UAB Research Foundation - Birmingham AL
The Trustees of The University of Pennsylvania - Philadelphia PA
The Board of Regents of The University of Washington - Seattle WA
The Trustees of The University of Pennsylvania - Philadelphia PA
The Board of Regents of The University of Washington - Seattle WA
International Classification:
C12N 1500, C12N 1585, C12N 1579
US Classification:
435 696
Abstract:
The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the. beta. -globin locus are sufficient to result in high level expression of human. alpha. - or. beta. -globin transgenes. The present invention also provides for novel recombinant nucleic acid vectors which may be used to produce. alpha. -globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the. beta. -globin locus together with a gene of interest. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human. alpha.
Synthesis Of Functional Human Hemoglobin And Other Proteins In Erythroid Tissues Of Transgenic Animals
US Patent:
5602306, Feb 11, 1997
Filed:
Jul 13, 1994
Appl. No.:
8/275313
Inventors:
Tim M. Townes - Birmingham AL
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Thomas M. Ryan - Birmingham AL
Richard D. Palmiter - Seattle WA
Ralph L. Brinster - Gladwyne PA
Richard R. Behringer - Philadelphia PA
Assignee:
The UAB Research Foundation - Birmingham AL
The Trustees of the University of Pennslyvania - Philadelphia PA
Board of Regents of the University of Washington - Seattle WA
The Trustees of the University of Pennslyvania - Philadelphia PA
Board of Regents of the University of Washington - Seattle WA
International Classification:
C12N 1500
US Classification:
800 2
Abstract:
The present invention relates to the synthesis of functional human hemoglobin and other proteins in erythroid tissues of transgenic non-human animals and erythroid cell lines. It is based on the discovery that two of the five hypersensitivity sites of the. beta. -globin locus are sufficient to result in high level expression of human. alpha. - or. beta. -globin transgenes. The present invention also provides for novel recombinant nucleic acid vectors which may be used to produce. alpha. -globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the. beta. -globin locus together with a gene of interest. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human. alpha.
Repopulation Of Testicular Seminiferous Tubules With Foreign Cells, Corresponding Resultant Germ Cells, And Corresponding Resultant Animals And Progeny
US Patent:
6215039, Apr 10, 2001
Filed:
Aug 26, 1998
Appl. No.:
9/140553
Inventors:
Ralph L. Brinster - Gladwyne PA
Assignee:
The Trustees of the University of Pennsylvania - Philadelphia PA
International Classification:
A01K 6700
US Classification:
800 8
Abstract:
An animal harboring a non-native germ cell, its corresponding line, and the corresponding germ cells, are obtained by colonizing the testis (or testes) of a host animal with primitive cells followed by raising and/or breeding the host.
Culture Conditions And Growth Factors Affecting Fate Determination, Self-Renewal And Expansion Of Rat Spermatogonial Stem Cells
US Patent:
2007019, Aug 16, 2007
Filed:
Sep 19, 2005
Appl. No.:
11/229985
Inventors:
Ralph Brinster - Gladwyne PA, US
Hiroshi Kubota - Swarthmore PA, US
Buom-Yong Ryu - Pyongtaek-Si, KR
Hiroshi Kubota - Swarthmore PA, US
Buom-Yong Ryu - Pyongtaek-Si, KR
International Classification:
A01K 67/027, G01N 33/567, C12N 5/06
US Classification:
800014000, 435007200, 435353000
Abstract:
The present invention relates to methods of identifying and enriching rat spermatogonioal stem cells, and compositions thereof. Further, the invention relates to methods and compositions for the isolation, maintenance and proliferation of rat spermatogonial stem cells, as well as methods and compositions for the identification and use of factors influencing rat spermatogonial stem cell maintenance and proliferation.
Repopulation Of Testicular Seminiferous Tubules With Foreign Cells, Corresponding Resultant Germ Cells, And Corresponding Resultant Animals And Progeny
US Patent:
5858354, Jan 12, 1999
Filed:
Nov 21, 1994
Appl. No.:
8/345738
Inventors:
Ralph L. Brinster - Gladwyne PA
Assignee:
The Trustees of the University of Pennsylvania - Philadelphia PA
International Classification:
A01N 6300, A61B 1743, C12N 506, C12N 1501
US Classification:
424 937
Abstract:
An animal harboring a non-native germ cell, its corresponding line, and the corresponding germ cells, are obtained by colonizing the testis (or testes) of a host animal with primitive cells followed by raising and/or breeding the host.
Techniques For Freezing Spermatogonia Cells
US Patent:
5817453, Oct 6, 1998
Filed:
May 31, 1996
Appl. No.:
8/658832
Inventors:
Ralph L. Brinster - Gladwyne PA
Assignee:
University of Pennsylvania - Philadelphia PA
International Classification:
A01N 102, C12N 500
US Classification:
435 11
Abstract:
Methods for cryopreserving spermatogonia cells are presented.