R. WILLIAMS, M.D.
Osteopathic Medicine at Erwin Rd, Durham, NC
License number
North Carolina 23347
Category
Osteopathic Medicine
Type
Cardiovascular Disease
Address
Address
2100 Erwin Rd, Durham, NC 27710
Phone
(919) 620-4467
Organization information
See more information about R. WILLIAMS at bizstanding.comAnthony R Williams
5318 Highgate Dr, Durham, NC 27713
Categories:
Attorneys
Phone:
(919) 667-2390 (Phone)
R Williams, MD
2100 Erwin Rd, Durham, NC 27705
Industry:
Internist
Phone:
(919) 620-4467 (Phone)
R A Williams Janitor
114 Lone Tree Ct, Durham, NC 27713
Industry:
Building Maintenance Services
Principal:
Robert Williams (Principal)
Professional information
Particle-Mediated Bombardment Of Dna Sequences Into Tissue To Induce An Immune Response
US Patent:
6194389, Feb 27, 2001
Filed:
Apr 11, 1997
Appl. No.:
8/840224
Inventors:
Stephen A. Johnston - Durham NC
R. Sanders Williams - Durham NC
John C. Sanford - Geneva NY
Sandra G. McElligott - Rose Valley PA
R. Sanders Williams - Durham NC
John C. Sanford - Geneva NY
Sandra G. McElligott - Rose Valley PA
Assignee:
Duke University - Durham NC
E.I. du Pont de Nemours & Company, Inc - Wilmington DE
Cornell Research Foundation, Inc. - Ithaca NY
E.I. du Pont de Nemours & Company, Inc - Wilmington DE
Cornell Research Foundation, Inc. - Ithaca NY
International Classification:
A61K 3170, C12N 1587
US Classification:
514 44
Abstract:
A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5' to 3' direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.
Transgenic Cardiomyocytes With Controlled Proliferation And Differentiation
US Patent:
2004000, Jan 1, 2004
Filed:
Mar 4, 2003
Appl. No.:
10/379375
Inventors:
Eric Olson - Dallas TX, US
David Markham - Durham NC, US
Igor Rybkin - Dallas TX, US
R. Williams - Durham NC, US
David Markham - Durham NC, US
Igor Rybkin - Dallas TX, US
R. Williams - Durham NC, US
Assignee:
Board of Regents, The University of Texas System
International Classification:
A01K067/027, C12N005/06
US Classification:
800/018000, 800/021000, 435/354000
Abstract:
The present invention provides methods for creating conditionally-immortal cell lines. These transgenic cell lines can be grown indefinitely in culture while maintaining a relatively undifferentiated stated. Upon appropriate switch signal, the cells cease replicating and differentiate much like adult cells. The switch is facilitated by the inactivation of a transforming gene, such as large T antigen. A convenient methodology for such inactivation is Cre-Lox mediated excision of the gene. Cardiac cells are provided as an example of useful a transgenic cell line.