Inventors:
Udeni B.R. Balasuriya - Lexington KY, US
Peter J. Timoney - Lexington KY, US
Jianqiang Zhang - Lexington KY, US
International Classification:
C12N 5/10, C07H 21/04, C12N 15/63, C07H 21/02
Abstract:
An isolated polynucleotide molecule includes a DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus, wherein the DNA sequence is SEQ ID NO:1 or a degenerate variant thereof. Also provided are transformed or transfected host cells including that sequence, vectors including the sequence, and isolated infectious RNA molecules encoded by the sequence. Further, a modified DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus is provided wherein the DNA sequence is SEQ ID NO:2 or a degenerate variant thereof, including a si lent point mutation allowing distinguishing the modified sequence from the parent and other strains of Equine arteritis virus.