Inventors:
Ji Zhang - Princeton Junction NJ
Peng Wang - Edison NJ
Assignee:
Schering Corporation - Kenilworth NJ
International Classification:
C12N 1587, C12N 1563
Abstract:
A baculovirus/insect cell expression system in which both. alpha. and. beta. subunit of human IL-5 receptor can be over-expressed together in Sf9 cells has been developed. This system is able to express the human high-affinity IL-5 receptors at a very high level and finds use in a screening assay for IL-5 receptor-based antagonists. A preferred embodiment of the screening assay involves the use of peanut agglutinin as lectin in a scintillation proximity assay.