PAUL K ADOLF
Veterinary in Easton, PA
License number
Pennsylvania BV007750L
Category
Veterinary Medicine
Type
Veterinarian
Address
Address
Easton, PA 18045
Professional information
Stable Colored Reference Standard For Enzymatic Determinations
US Patent:
4056485, Nov 1, 1977
Filed:
Feb 4, 1976
Appl. No.:
5/655180
Inventors:
Paul K. Adolf - Easton PA
James J. Carroll - East Hanover NJ
James J. Carroll - East Hanover NJ
Assignee:
Warner-Lambert Company - Morris Plains NJ
International Classification:
C09K 300, G01N 3106, G01N 3122
US Classification:
252408
Abstract:
A stable, colored reference standard suitable for use in diagnostic determinations involving enzymatic reactions in which colorless 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride is reduced to the red-colored 1-(p-iodophenyl)-5-(p-nitrophenyl)-3-phenylformazan, is described. The reference standard of this invention is an aqueous solution containing, in specified amounts, 1-(p-iodophenyl)-5-(p-nitrophenyl)-3-phenylformazan; serum albumin; an N,N'-dimethylformamide or a dimethylsulfoxide solvent; and isopropyl alcohol. An inert bulking agent may be added to the aqueous colored standard and the solution obtained may then be lyophilized. The lyophilized form of the colored reference standard is stable for at least 3 months at 4. degree. C. and 24. degree. C. and can be easily reconstituted with water or with dilute aqueous hydrochloric acid solutions. The novel colored reference standard solution has an absorbence maximum of 500 nanometers.