Inventors:
Patricia A. Harder - Wilmington DE
Joan T. Odell - Unionville PA
Assignee:
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
C12N 504, C12N 1529, C12N 1582, C12N 1590, A01H 500
Abstract:
This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a soybean homolog of the Phaseolus vulgaris PvAlf transcription activator. The invention also relates to the construction of chimeric genes encoding all or a portion of a soybean homolog of the Phaseolus vulgaris PvAlf transcription activator, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of a plant homolog of the Phaseolus vulgaris PvAlf transcription activator in a transformed host cell. The invention also relates to targeting of the soybean homolog of the Phaseolus vulgaris PvAlf transcription activator to a novel promoter region by the addition of either a DNA-binding domain or a protein-protein interaction domain, thus leading to a higher level of gene expression.