OLIVER ELON OWEN
Medical Practice in Gladwyne, PA
License number
Pennsylvania MD010610E
Category
Medicine
Type
Medical Physician and Surgeon
Address
Address
Gladwyne, PA 19035
Personal information
See more information about OLIVER ELON OWEN at radaris.comName
Address
Phone
1401 Spring Mill Rd, Gladwyne, PA 19035
Professional information
Semi-Quantitative Assay Of Lactic Acid And .Beta.-Hydroxy Butyrate
US Patent:
4254222, Mar 3, 1981
Filed:
Jul 19, 1978
Appl. No.:
6/925946
Inventors:
Oliver E. Owen - Gladwyne PA
International Classification:
C12Q 132
US Classification:
435 26
Abstract:
There is provided a procedure for determining the concentration of a metabolic acid in a biological fluid. The method involves use of a tetrazolium salt, a pyridine nucleotide, an electron carrier, and an enzyme that is a dehydrogenase for the specific acid being assayed. These components are used to form an assay mixture; thereafter a quantity of the fluid to be assayed is combined with the assay mixture, so that there may commence a reaction in which the tetrazolium salt is changed to a formazan in an amount that is indicative of the concentration of the specific acid. In a preferred embodiment, the procedure permits assay of the concentration of beta-hydroxybutyrate, and the assay mixture includes components in the relative proportions of 0. 8 micromoles 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT), 0. 32 micromoles phenazine methosulfate (PMS), 1.
Semi-Quantitative Assay Of Metabolic Acids
US Patent:
4351899, Sep 28, 1982
Filed:
Feb 19, 1981
Appl. No.:
6/235776
Inventors:
Oliver E. Owen - Gladwyne PA
International Classification:
C12Q 132
US Classification:
435 26
Abstract:
A test indicator determines concentration, in a biological fluid, of a metabolic acid, such as beta-hydroxybutyrate or lactic acid. The indicator includes a test surface containing the dried residue resulting from impregnation of the surface with components including a tetrazolium salt, nicotinamide adenine dinucleotide (NAD), an electron carrier, and the enzyme that is a dehydrogenase for the specific metabolic acid being assayed. The indicator also includes a physically distinct provision for buffering the reaction. In accordance with the concentration of the metabolic acid being assayed, the indicator provides a predetermined color or intensity which can be observed either with the naked eye or with instrumentation such as a reflectance meter.