OILDA RUBIO
Medical Practice at 18 St, Miami, FL
License number
Florida 28024
Issued Date
Dec 22, 1988
Effective Date
Jul 25, 2008
Expiration Date
Aug 31, 2018
Category
Health Care
Type
Clinical Laboratory Personnel
Address
Address
13710 SW 18Th St, Miami, FL 33175
Professional information
Staff Quality Assurance Scientist At Beckman Coulter Inc
Position:
Staff Applications Scientist at Beckman Coulter
Location:
Miami/Fort Lauderdale Area
Industry:
Medical Devices
Work:
Beckman Coulter
- Miami, FL since 1990
-
Staff Applications Scientist
Education:
Florida International University 2000
Master's degree, Biomedical Sciences, General Rutgers University
Bachelor of Science (BS), Medical Technolgy/Biology
Master's degree, Biomedical Sciences, General Rutgers University
Bachelor of Science (BS), Medical Technolgy/Biology
Interests:
Art, Drawing with Charcoal and Graphite.
Method For A Fully Automated Monoclonal Antibody-Based Extended Differential
US Patent:
7625712, Dec 1, 2009
Filed:
May 17, 2005
Appl. No.:
11/130492
Inventors:
Ronald D. Paul - Fort Lauderdale FL, US
James L. Wyatt - San Diego CA, US
Barbara Carrillo - Miami FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
James L. Wyatt - San Diego CA, US
Barbara Carrillo - Miami FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
G01N 33/567
US Classification:
435 721, 435 723, 435 724, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207
Abstract:
A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.
Method For A Fully Automated Monoclonal Antibody-Based Extended Differential
US Patent:
7674598, Mar 9, 2010
Filed:
Jun 27, 2006
Appl. No.:
11/475313
Inventors:
Ronald D. Paul - Fort Lauderdale FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
Ravindra Mylvaganam - Longmeadow MA, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
Ravindra Mylvaganam - Longmeadow MA, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
G01N 33/567
US Classification:
435 724, 435 71, 435 721, 435 723, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207
Abstract:
Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.
Method And Apparatus For Preparing Cell Samples For Intracellular Antigen Detection Using Flow Cytometry
US Patent:
2004022, Nov 18, 2004
Filed:
May 14, 2003
Appl. No.:
10/437695
Inventors:
Oilda Rubio - Miami FL, US
Carlos Aparicio - Miami FL, US
John Maples - Miami FL, US
Julie Wilkinson - Fort Lauderdale FL, US
Cecilia Smith - Miami FL, US
Frank Lucas - Boca Raton FL, US
Carlos Aparicio - Miami FL, US
John Maples - Miami FL, US
Julie Wilkinson - Fort Lauderdale FL, US
Cecilia Smith - Miami FL, US
Frank Lucas - Boca Raton FL, US
Assignee:
Beckman Coulter, Inc. - Fullerton CA
International Classification:
G01N033/48
US Classification:
436/063000, 422/073000
Abstract:
A method and apparatus for preparing biological cell samples for intracellular analysis. The invention is based upon the recognition that many of the steps of the conventional methods for such sample preparation can be eliminated, leading to a process that readily lends itself to automation and the advantages associated therewith. The method of the invention comprises the steps of (a) cell-fixation, (b) permeabilization and (c) staining (or labeling) of intracellular molecules of interest by probes that are readily detectable by flow cytometric techniques, all without any intervening cell-washing (and re-suspension) steps. Rather, the single cell-washing step is effected after these three steps have been carried out. Preferably, the washing step is carried out by passing the fixed, permeabilized and stained cell sample through a semi-permeable membrane that serves to filter out (by transmission) interferants to waste while retaining the cells of interest.