Work:
Institute and Joint Center for Structural Genomics
Research Associate (Prof. Scott Lesley, Principle Investigator)
Stony Brook University - Stony Brook, NY
Mentor
Department of Chemistry - Stony Brook, NY
Research Assistant (Prof. Peter J. Tonge, Principal Investigator)
Department of Chemistry, Stony Brook University
Teaching Assistant
Nanjing University - Nanjing, China
Research Assistant
Education:
The Scripps Research Institute and Joint Center
Research Associate
Stony Brook University - Stony Brook, NY
Doctor of Philosophy in Chemistry & Biochemistry
Skills:
Enzymology and Assay Development Enzyme kinetics, enzymatic reaction mechanism determination, inhibition mechanism determination, steady state kinetics, pre-steady state enzyme kinetics, protein-ligand binding affinity determination, ligand residence time determination, MIC determination UV-vis, fluorescence, ITC, DSC, SPR, radioligand binding assay, filter binding assay, gel shift assay, dynamic light scattering instrument, HPLC, and high-throughput assay development Molecular Biology Genomic database mining, primer design, PCR, RT-PCR, clone, site-directed mutagenesis, DNA isolation and purification, site-specific chromosomal mutagenesis, gene knockout, in vitro translation, Western blot, and high-throughput technology. Protein Expression and Purification Recombinant protein expression in bacterial and mammalian system, protein-protein complex coexpression, unnatural amino acid incorporation, site-specific protein modification and high-throughput expression Protein purification with KTA Explorer, Metal affinity chromatography (MAC), Size-exclusion chromatography (SEC), Ion exchange chromatography, dialysis, centrifugation, purification from inclusion body and high-throughput purification. Protein Characterization SDS/Native-PAGE, LabChip 90, Western blot, Bradford assay and BCA assay, analytical-SEC, analytical ultracentrifugation (AUC), MALDI-MS, ESI-MS, Circular Dichroism (CD) and high-throughput characterization Protein Crystallography Protein-small molecule and protein-protein complex crystallization, liquid handling equipment, robotic crystallization setup, cryo crystal mounting, structural determination and high-throughput crystallization Soft Skills and Softwares Team work, problem-solving, multitask management, excellent oral presentation Microsoft Office (Word, Excel, Powerpoint), ChemOffice, SciFinder, Endnot, Sigmaplot, GraFit, GraphPad CoralDraw, PyMOL, LINUX, HKL2000, CCP4, MIFit and Coot.