NICHOLAS DAVID TONKS
Pilots at Arrowhead Pl, Hunt, NY
License number
New York A5105303
Issued Date
Jul 2016
Expiration Date
Jul 2017
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
3 Arrowhead Pl, Hunt, NY 11743
Professional information
Isolation Of A Cdna Encoding A Novel Protein Tyrosine Phosphatase Which Localizes To Focal Adhesions And Uses Therefor
US Patent:
2003011, Jun 19, 2003
Filed:
Nov 12, 2002
Appl. No.:
10/293231
Inventors:
Nicholas Tonks - Huntington NY, US
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
A61K048/00, C07H021/02, C12N007/00, C12N015/867
US Classification:
424/093200, 435/456000, 435/320100, 435/235100, 536/023100
Abstract:
DNA which encodes a protein homologous to the protein tyrosine phosphatases (PTPases) which catalyze the dephosphorylation of proteins in which tyrosyl residues have been phosphorylated through the action of a protein kinase. The protein, which appears as if it will localize to focal adhesions, is also the subject of the present invention. In particular, it relates to CDNA encoding a protein, referred to as PTPH1. Overexpression of PTPH1 may be a powerful approach to countering the effects of oncogenic protein tyrosine kinases, such as those of transforming viruses, and interfering with or reversing cell transformation. This would provide a means of preventing or reversing abnormally high levels of phosphotyrosine associated with any diseases or condition, such as preventing or reversing malignancy associated with the activity of a protein tyrosine kinase. In the present method, DNA or RNA encoding PTPH1 or a functional equivalent of PTPH1 is administered to an individual in an appropriate gene transfer vehicle which can infect mammalian cells and, once inside the mammalian cells, express and make available PTPH1 or its functional equivalent in sufficient quantities to overcome or counteract the protein tyrosine kinase activity. As a result, phosphorylation of tyrosine residues at abnormal levels is prevented or reversed, resulting in turn in prevention or reversal of malignancy of cells.
Density Enhanced Protein Tyrosine Phosphatases
US Patent:
6552169, Apr 22, 2003
Filed:
Nov 23, 1999
Appl. No.:
09/447533
Inventors:
Nicholas K. Tonks - Huntington NY
Arne Ostman - Uppsala, SE
Arne Ostman - Uppsala, SE
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
C07K 1447
US Classification:
530350, 536 232, 435 691, 435325, 4353201, 435 711, 435 712, 435471, 4352523, 43525411, 435196
Abstract:
Novel Type III density enhanced protein tyrosine phosphatases are disclosed and exemplified by human DEP-1 enzyme. Polynucleotides encoding huDEP-1 are disclosed, along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for DEP-1 are also disclosed as useful for modulating the biological activities of DEP-1.
Reversible Oxidation Of Protein Tyrosine Phosphatases
US Patent:
2003021, Nov 20, 2003
Filed:
Feb 12, 2003
Appl. No.:
10/366547
Inventors:
Tzu-Ching Meng - Oyster Bay NY, US
Nicholas Tonks - Huntington NY, US
Deborah Cool - Snohomish WA, US
Nicholas Tonks - Huntington NY, US
Deborah Cool - Snohomish WA, US
International Classification:
C12Q001/42
US Classification:
435/021000
Abstract:
The invention relates to a method of identifying any protein tyrosine phosphatase (PTP) that undergoes reversible modification of PTP active site invariant cysteine within a cell, such that the phosphatase is transiently protected from irreversible active site invariant cysteine-directed PTP inactivating agents. Methods related to regulation of PTPs by reactive oxygen species (ROS) in a cellular environment are provided. Multiple PTPs are shown to be reversibly oxidized and inactivated following treatment of cells with HOor with physiological stimuli that promote ROS formation, and inhibition of PTP function is shown to contribute to ROS-induced mitogenesis. Transient oxidation of the PTP catalytic site invariant cysteine is exploited in methods to identify which of multiple candidate PTPs are components of a given biological signal transduction pathway, without a requirement for first specifically purifying any particular candidate PTP.
Anticancer And Immunomodulating Molecules And Fractions Containing Said Molecules, And Process For Preparing Said Fractions And Said Molecules From Fermented Vegetal Material, And Their Uses
US Patent:
2012016, Jun 28, 2012
Filed:
Jul 11, 2011
Appl. No.:
13/135616
Inventors:
Mate Hidvegi - Budapest, HU
Gyula Bencze - Budapest, HU
Gyorgy Keri - Budapest, HU
Laszlo Orfi - Budapest, HU
Darryl Pappin - Boxborough MA, US
Nicholas K. Tonks - Huntington NY, US
James Watson - Cold Spring Harbor NY, US
Gyula Bencze - Budapest, HU
Gyorgy Keri - Budapest, HU
Laszlo Orfi - Budapest, HU
Darryl Pappin - Boxborough MA, US
Nicholas K. Tonks - Huntington NY, US
James Watson - Cold Spring Harbor NY, US
International Classification:
A61K 36/899, A61P 37/00, A61P 3/00, A61P 35/00
US Classification:
424123, 424115
Abstract:
Biologically active substances and fractions of wheat germ ferment/fermented wheat germ extract (FWGE), including formulation A250, the processes for their production, the pharmaceutical preparations containing them, and their uses.
Dep-1 Receptor Protein Tyrosine Phosphatase Interacting Proteins And Related Methods
US Patent:
2008002, Jan 31, 2008
Filed:
Jan 26, 2007
Appl. No.:
11/698390
Inventors:
Helena Palka-Hamblin - Chicago IL, US
Nicholas Tonks - Huntington NY, US
Nicholas Tonks - Huntington NY, US
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
C12N 9/20, C07H 21/04, C12P 21/06
US Classification:
435069100, 435198000, 435320100, 435325000, 536023200
Abstract:
Proteins are identified from human breast tumor cell lines (MDA-MB-231, T-47D and T-47D/Met) that interact specifically with the substrate-trapping mutant form of Density Enhanced Phosphatase-1 (DEP-1). These proteins include the functional component p120 catenin (p120), the adaptor protein Gab 1, and the HGF/SF receptor Met. The invention relates to isolated complexes comprising DEP-1 polypeptides in specific association with Met, Gab 1, or p120, identified herein as DEP-1 substrate polypeptides. Screening assays for agents that alter DEP-1 interaction with DEP-1 substrate polypeptides are also disclosed, as are methods for altering biological signals in cells that are transduced via DEP-1 pathways.
Transcriptional Regulation Of Ptp-1B
US Patent:
2003022, Dec 4, 2003
Filed:
Mar 11, 2003
Appl. No.:
10/388215
Inventors:
Nicholas Tonks - Huntington NY, US
Toshiyuki Fukada - Huntington NY, US
Toshiyuki Fukada - Huntington NY, US
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
A61K048/00, C12Q001/68, C07H021/04, C12N009/16, C12P021/02, C12N005/06
US Classification:
424/093210, 514/044000, 435/006000, 435/320100, 435/325000, 435/196000, 435/069100, 536/023200
Abstract:
Compositions and methods relating to PTP1B associated disorders are provided, based on the discovery that a Y-box protein binding site is present as a transcription enhancer sequence in the promoter region situated upstream (i.e., 5′ to) of the human PTP1B gene. This site, situated at nucleotides -155 through -132 of the human PTP1B gene, mediates specific binding interactions with the YB-1 transcription regulatory factor, a member of the Y-box family of proteins. YB-1-targeted antisense constructs reduced PTP1B expression levels, providing an alternative to PTP1B active-site directed regulation of PTP1B activity.
Substrate Trapping Protein Tyrosine Phosphatases
US Patent:
5912138, Jun 15, 1999
Filed:
Jul 25, 1996
Appl. No.:
8/685992
Inventors:
Nicholas Tonks - Huntington NY
Andrew J. Flint - Bothell WA
Andrew J. Flint - Bothell WA
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
C12N 916, C12Q 142
US Classification:
435 21
Abstract:
Novel protein tyrosine phosphatases in which the invariant aspartate residue is replaced with an alanine residue and which bind to a tyrosine phosphorylated substrate and are catalytically attenuated are described. Also described are methods of identifying tyrosine phosphorylated proteins which complex with the described protein tyrosine phosphatases.
Substrate Trapping Protein Tyrosine Phosphatases
US Patent:
5951979, Sep 14, 1999
Filed:
Sep 1, 1998
Appl. No.:
9/144925
Inventors:
Nicholas Tonks - Huntington NY
Andrew J. Flint - Bothell WA
Andrew J. Flint - Bothell WA
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
A61K 3846, C12N 916
US Classification:
424 946
Abstract:
Novel protein tyrosine phosphatases in which the invariant aspartate residue is replaced with an alanine residue and which bind to a tyrosine phosphorylated substrate and are catalytically attenuated are described. Also described are methods of identifying tyrosine phosphorylated proteins which complex with the described protein tyrosine phosphatases.
Dna Encoding Density Enhanced Protein Tyrosine Phosphatases
US Patent:
6114140, Sep 5, 2000
Filed:
May 12, 1997
Appl. No.:
8/854585
Inventors:
Nicholas K. Tonks - Huntington NY
Arne Ostman - Uppsala, SE
Arne Ostman - Uppsala, SE
Assignee:
Cold Spring Harbor Laboratory
International Classification:
C12N 1552, C12N 1563, C12N 510
US Classification:
435 691
Abstract:
Novel Type III density enhanced protein tyrosine phosphatases are disclosed and exemplified by human DEP-1 enzyme. Polynucleotides encoding huDEP-1 are disclosed, along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for DEP-1 are also disclosed as useful for modulating the biological activities of DEP-1.
Density Enhanced Protein Tyrosine Phosphatases
US Patent:
7195762, Mar 27, 2007
Filed:
Mar 13, 2003
Appl. No.:
10/390501
Inventors:
Nicholas K Tonks - Huntington NY, US
Arne Ostman - Uppsala, SE
Arne Ostman - Uppsala, SE
Assignee:
Cold Spring Harbor Laboratory - Cold Spring Harbor NY
International Classification:
A61K 39/395
US Classification:
4241331, 4241431, 435326
Abstract:
Novel Type III density enhanced protein tyrosine phosphatases are disclosed and exemplified by human DEP-1 enzyme. Polynucleotides encoding huDEP-1 are disclosed, along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for DEP-1 are also disclosed as useful for modulating the biological activities of DEP-1.