MINH HANH THI NGUYEN
Pharmacy at Pala Mesa Dr, San Jose, CA
License number
California RPH 50524
Category
Pharmacy
Type
Pharmacist
Address
Address
5847 Pala Mesa Dr, San Jose, CA 95123
Phone
(408) 793-2750
(408) 793-2751 (Fax)
(408) 793-2751 (Fax)
Personal information
See more information about MINH HANH THI NGUYEN at radaris.comName
Address
Phone
20514 Gault St, Winnetka, CA 91306
3376 Golfers Dr, Oceanside, CA 92056
525 E Maude Ave APT 31, Sunnyvale, CA 94085
6320 Bataan St, Cypress, CA 90630
3965 Nobel Dr #102, San Diego, CA 92122
Professional information
Primer Beads
US Patent:
2013027, Oct 17, 2013
Filed:
Jun 30, 2011
Appl. No.:
13/805498
Inventors:
Nam Quoc Ngo - Los Gatos CA, US
Hoc Thai Nguyen - Bac Lieu Province, VN
Minh Tri Thi Dang - San Jose CA, US
Ngoc Dieu Ngo - San Jose CA, US
Laurent Jaquinod - Davis CA, US
Hoc Thai Nguyen - Bac Lieu Province, VN
Minh Tri Thi Dang - San Jose CA, US
Ngoc Dieu Ngo - San Jose CA, US
Laurent Jaquinod - Davis CA, US
Assignee:
Chemistry and Technology For Genes, Inc. - San Jose CA
International Classification:
C12P 19/34
US Classification:
435 912, 435262, 435194
Abstract:
Non-hydrophobic beads and methods to reversibly bind, normalize, store and in situ deliver primers to reactions including PCR. Also provided are instructions for preparing the beads. In the presence of an appropriate binding buffer, a bead can be used to bind and desalt primers from a crude solution of DMT-off primers. In the presence of an appropriate binding buffer, a bead can be used to bind and purify primers from a crude solution of DMT-on primers. A bead may bind a picomolar amount of DMT-on primers from a solution containing a plurality of crude DMT-on primers. Upon detritylation and washing, the resulting DMT-off primer bound bead may be used in PCR. Primers are released from the bead upon cycling the temperature. Primer bound beads are coated or silanized with hydrophobic reagents which ensures a gradual release of primers during the thermal cycling of the PCR reaction. Coating or silanization in turn enhances primer stability and long term storage.