Inventors:
Cheryl A. Nickerson - Phoenix AZ, US
James W. Wilson - Wilmington DE, US
Mark C. Ott - Houston TX, US
Eric A. Nauman - West Lafayette IN, US
Michael J. Schurr - Aurora CO, US
Shameema Sarker - Chandler AZ, US
Assignee:
Arizona Board of Regents, for and on behalf of Arizona State University - Tempe AZ
International Classification:
A61K 49/00, A61K 39/112, C12Q 1/68, G01N 33/53, C12Q 1/02, C12Q 1/18, C12N 1/20, C12N 1/18, C12N 1/16, C40B 30/04
US Classification:
424 92, 4242351, 435 6, 435 71, 435 29, 435 32, 4352528, 4352533, 4352534, 4352552, 43525521, 4352554, 506 9
Abstract:
This invention is directed to applying a low sedimental fluid shear environment to manipulate microorganisms, and to microorganisms and compositions obtained based on such manipulation. Specifically, the present invention provides methods of modifying a molecular genetic or phenotypic characteristic (e.g., virulence, stress resistance or biofilm formation) of a microorganism by culturing in a low sedimental shear environment. One or more ion concentrations in the culture can be modulated in order to inhibit or amplify the extent of the modification. The present invention also provides microorganisms obtained from a low sedimental shear culture, which exhibit modified and desirable phenotypic characteristics, as well as therapeutic, vaccine and bioindustrial products prepared from such microorganisms. Further, the present invention provides methods for identifying molecules that modulate responses of a microorganism to a low sedimental shear environment and for determining the relevance of such molecules to pathogenenicity of the microorganism.