Inventors:
Peter B. Vander Horn - Encinitas CA, US
Cheng-Yao Chen - Carlsbad CA, US
Guobin Luo - Oceanside CA, US
Michael Previte - Carlsbad CA, US
Jamshid Temirov - Germantown TN, US
Theo Nikiforov - Carlsbad CA, US
Zhaohui Zhou - San Ramon CA, US
Hongye Sun - Belmont CA, US
Yufang Wang - San Carlos CA, US
Stefanie Yukiko Nishimura - Mountain View CA, US
Hongyi Wang - Pearland TX, US
Marian Peris - Belmont CA, US
Barnett B. Rosenblum - San Jose CA, US
Michael Phelan - Hayward CA, US
Assignee:
Life Technologies Corporation - Carlsbad CA
International Classification:
C12Q 1/68
Abstract:
Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.