DR. MICHAEL HAFFNER, M.D.
Medical Practice in Baltimore, MD
License number
Maryland X
Category
Medical Practice
Type
Anatomic Pathology
Address
Address
600 N Wolfe St Pathology Bldg., Baltimore, MD 21287
Phone
(410) 955-3439
(443) 851-7730 (Fax)
(443) 851-7730 (Fax)
Personal information
See more information about MICHAEL HAFFNER at radaris.comName
Address
Phone
960 Fell St UNIT 303, Baltimore, MD 21231
Professional information
5-Hydroxymethylcytosine In Human Cancer
US Patent:
2014003, Feb 6, 2014
Filed:
Aug 1, 2013
Appl. No.:
13/956879
Inventors:
Michael Christoph Haffner - Baltimore MD, US
Alcides Chaux - Baltimore MD, US
William G. Nelson - Towson MD, US
Angelo M. DeMarzo - Baltimore MD, US
Alcides Chaux - Baltimore MD, US
William G. Nelson - Towson MD, US
Angelo M. DeMarzo - Baltimore MD, US
Assignee:
The Johns Hopkins University - Baltimore MD
International Classification:
G01N 33/574
US Classification:
435 611
Abstract:
The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.