MICHAEL H BERMAN
Engineers in Rockville, MD

4987073, Jan 22, 1991

Mar 14, 1990

7/494844

Michael L. Berman - Potomac MD
P. Scott Meissner - Barnesville MD
William P. Sisk - Kensington MD

Akzo N.V. - Arnhem

C12P 1934, C12N 1500, C07H 1512

4351723

A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple and efficient cDNA cloning system reduces the amount of RNA and effort required for the preparation of large directionally cloned libraries by providing a unique vector that accepts cDNA fragments in a unique 5'-3' orientation and by providing a unique bifunctional linker that facilitates the preparation of cDNA fragments that can be ligated directionally into the vector.

5338832, Aug 16, 1994

Aug 13, 1992

7/929842

Nicholas Pomato - Frederick MD
Ebo S. Bos - De Veiesstratt, NL
Martin V. Haspel - Silver Spring MD
Michael G. Hanna - Frederick MD
Michael L. Berman - Potomac MD

Akzo N.V. - Velperweg

C07K 1300, C07K 500, C07K 1528

530350

The present invention comprises the epitope recognized by the human monoclonal antibody 16-18, the human tumor antigen containing this epitope, which we have identified, isolated and characterized, and anti-idiotypic antibodies to human MCA 16-88, which comprise the same epitope. The invention also relates to the use of antibodies to the antigen containing this epitope for diagnosis and monitoring of treatment of cancer and to the use of this antigen in the preparation of vaccines to elicit an immune response similar to that obtained against tumor cells containing this epitope.