Inventors:
Michael P. Farrell - Marlborough MA
Juili L. Lin-Goerke - Framingham MA
International Classification:
C12Q 168, C12P 1930, C07H 2102
Abstract:
Disclosed herein is a composition comprising ribonuclease treated RNA or DNA dependent RNA polymerase, and the use of same in amplification methods. The treatment of the RNA or DNA dependent RNA polymerase with ribonuclease reduces or eliminates false positives which result from the presence of an endogenous or contaminating replicatable template species in the Q. beta. replicase enzyme preparation.