Inventors:
David R. Blanco - Beverly Hills CA
James N. Miller - Northbridge CA
Michael A. Lovett - Los Angeles CA
Cheryl I. Champion - Culver City CA
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
C07K 100
US Classification:
530412, 530350, 435 691, 435 6, 435 911, 435 915, 435170, 435 795, 435 71, 536 231, 536 243, 4242621, 514263
Abstract:
Antigenic rare outer membrane proteins of Spirochaetaceae are obtained from organisms extracted from infected tissue by a novel process of isolation utilizing a discontinuous Ficoll gradient separation, release of outer membrane in a low isotonic and low pH buffer and identification of outer membrane by use of a lipid soluble dye. Four antigenic rare outer membrane proteins of subsp. useful in diagnosis and prophylaxis of syphilis are provided. Also provided is the amino acid sequence of a rare outer membrane protein of subsp. and the nucleotide sequence encoding it.