Inventors:
Heather Desaire - Lawrence KS, US
Jennifer Laurence - Lawrence KS, US
Melinda Toumi - Lawrence KS, US
Jamie Wenke - Nashville TN, US
International Classification:
C07K 14/61, C12P 21/00, C07H 21/04, C12N 15/00, C07K 1/22
US Classification:
530397, 435 681, 536 231, 435455, 530395
Abstract:
Methods of producing and purifying proteins that comprise a glycan tag are provided. In some embodiments, a protein may be modified to include a glycan tag so as to facilitate production of the protein, e.g., by promoting protein secretion and/or promoting protein solubility. In some embodiments, the present disclosure provides methods wherein a protein may be modified to include a glycan tag, which may then be used as an affinity tag for purification.