Inventors:
Charles Henry - Fort Collins CO, US
Matthew Feirer - Fort Collins CO, US
Meghan Mensack - Thornton CO, US
Assignee:
COLORADO STATE UNIVERSITY RESEARCH FOUNDATION - Fort Collins CO
International Classification:
B01L 3/00
Abstract:
The present invention provides devices and associated methods to facilitate multiplexed staining on a slide. The use of multiplexed staining on a single slide allows for more accurate disease diagnosis while also conserving sample. A PDMS based cytology overlay was created that is capable of coupling on-chip morphological, cytochemical, and immunological staining. Morphological and cytochemical staining of blood smears, along with immunostaining of lymph node imprints, was carried out to demonstrate the application of this microfluidic cytology chip (μCC) device.