DR. MATTHEW KENT TOPHAM, M.D.
Osteopathic Medicine at Circle Of Hope Dr, Salt Lake City, UT

7241570, Jul 10, 2007

Mar 22, 2002

10/471122

Stephen M. Prescott - Salt Lake City UT, US
Matthew Topham - Salt Lake City UT, US
Fumio Sakane - Sapporo, JP
Akinobu Taketomi - Nakatsu, JP

University of Utah Research Foundation - Salt Lake City UT

C12Q 1/48, C12Q 1/68

435 6, 435 15

The present invention provides a method of screening for agents which may regulate or inhibit the activities of TACE and TGF-α. These agents may act by enhancing or inhibiting the activity of DGK-δ. An activity of DGK-δ is the enzymatic conversion of DAG to PA. The method includes contacting a cell or organism with a test agent determining the activity of DGK-δ within the cell. An agent with reduces or increases the activity of DGK may be used to regulate or inhibit the activity of TACE and TGF-α. Such agents can be used to treat diseases such as cancer where cell growth and division and inflamation are important factors.

2004013, Jul 8, 2004

Sep 8, 2003

10/471116

Stephen Prescott - Salt Lake City UT, US
Matthew Topham - Salt Lake City UT, US
Nicolas Bazan - New Orleans LA, US
Elena B Rodriguez de Turco - New Orleans LA, US

A61K049/00, C12Q001/48

435/015000, 424/009200

The present invention includes the characterization of the DGKe gene and the generation of screening methods for compounds that inhibit the function of DGKe. The DGK family of enzymes occupies a signaling crossroads since they catalyze the phosphorylation of DAG to produce PA. Both the substrate (DAG) and the product (PA) of this reaction are key factors in intracellular signaling, making the regulation of DGKe activity important to understand and control. DGKe -/- mice were also generated and studied to assist in understanding the function of DGKs in regulating cellular signaling. DGKe displays selectively for 20:4-DAG and is highly expressed in different areas of the brain, including Purkinje cells in the cerebellum, hippocampal interneurons, and the Pyramidal neurons in the CA3 region of the hippocampus.

2007016, Jul 12, 2007

Feb 23, 2007

11/710067

Stephen Prescott - Solt Lake City UT, US
Matthew Topham - Salt Lake City UT, US
Nicolas Bazan - New Orleans LA, US
Elena Rodriguez de Turco - New Orleans LA, US

A61K 31/66, C12Q 1/48

435015000, 514102000

The present invention includes the characterization of the DGKε gene and the generation of screening methods for compounds that inhibit the function of DGKε. The DGK family of enzymes occupies a signaling crossroads since they catalyze the phosphorylation of DAG to produce PA. Both the substrate (DAG) and the product (PA) of this reaction are key factors in intracellular signaling, making the regulation of DGKε activity important to understand and control. DGKε −/− mice were also generated and studied to assist in understanding the function of DGKs in regulating cellular signaling. DGKε displays selectively for 20:4-DAG and is highly expressed in different areas of the brain, including Purkinje cells in the cerebellum, hippocampal interneurons, and the Pyramidal neurons in the CA3 region of the hippocampus.

5976875, Nov 2, 1999

Apr 22, 1997

8/841483

Stephen M. Prescott - Salt Lake City UT
Michaeline Bunting - Salt Lake City UT
Wen Tang - Salt Lake City UT
Matthew Topham - Salt Lake City UT

University of Utah - Salt Lake City UT

C12N 120, C07H 2104

435325

Two novel diacylglycerol kinase (DGK) isoforms are disclosed. The cDNA of one DGK isoform, designated DGK. epsilon. , is about 2. 6 kb in length. The DGK. epsilon. cDNA has an open reading frame encoding 567 amino acids and has a predicted molecular mass of 63 kDA. DAG kinase. epsilon. is highly selective for arachidonate-containing diacylglycerol (DAG) substrates. The cDNA of the second isoform, designated DGK. zeta. , is 3. 5 kb in length. The DGK. zeta. cDNA contains a single large open reading frame encoding a 928-amino acid protein with a predicted molecular mass of 103. 9 kDa. An alternatively spliced muscle specific species of DGK. zeta. , DGK. zeta. -2 is also disclosed and characterized. DGK. zeta. is localized to the nucleus. A lysine-rich region with homology to the MARCKS protein is shown to be necessary and sufficient to confer nuclear localization to DGK. zeta.

6221658, Apr 24, 2001

Aug 25, 1999

9/382911

Stephen M. Prescott - Salt Lake City UT
Michaeline Bunting - Salt Lake City UT
Wen Tang - Salt Lake City UT
Matthew Topham - Salt Lake City UT

University of Utah Research Foundation - Salt Lake City UT

C12N 502, C12N 120, C07H 2104

435325

Two novel diacylglycerol kinase (DGK) isoforms are disclosed. The cDNA of one DGK isoform, designated DGK. epsilon. , is about 2. 6 kb in length. The DGK. epsilon. cDNA has an open reading frame encoding 567 amino acids and has a predicted molecular mass of 63 kDA. DAG kinase. epsilon. is highly selective for arachidonate-containing diacylglycerol (DAG) substrates. The cDNA of the second isoform, designated DGK. zeta. , is 3. 5 kb in length. The DGK. zeta. cDNA contains a single large open reading frame encoding a 928-amino acid protein with a predicted molecular mass of 103. 9 kDa. An alternatively spliced muscle specific species of DGK. zeta. , DGK. zeta. -2 is also disclosed and characterized. DGK. zeta. is localized to the nucleus. A lysine-rich region with homology to the MARCKS protein is shown to be necessary and sufficient to confer nuclear localization to DGK. zeta.