Inventors:
David Baltimore - Pasadena CA, US
Matthew Porteus - Dallas TX, US
International Classification:
C12Q001/68, C07H021/04, C12N009/22
US Classification:
435006000, 435069100, 435199000, 435320100, 435325000, 536023200
Abstract:
Gene targeting is a technique to introduce genetic change into one or more specific locations in the genome of a cell. For example, gene targeting can introduce genetic change by modifying, repairing, attenuating or inactivating a target gene or other chromosomal DNA. In one aspect, this disclosure relates to methods and compositions for gene targeting with high efficiency in a cell. This disclosure also relates to methods of treating or preventing a genetic disease in an individual in need thereof. Further disclosed are chimeric nucleases and vectors encoding chimeric nucleases.