Inventors:
Robert M. Corn - Madison WI, US
Emily A. Smith - Madison WI, US
Bernard Weisblum - Madison WI, US
Matthew G. Erickson - Madison WI, US
Andrew T. Ulijasz - Madison WI, US
Matthew J. Wanat - Madison WI, US
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
G01N 33/00, G01N 33/53, C07C 319/02
US Classification:
436120, 436119, 435 4, 435 71, 435 72, 435DIG 49, 568 18, 568 29
Abstract:
Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an ω-modified alkanethiol monolayer to the substrate and then contacting the ω-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.