MARTIN DAVID JOHNSON
Pilots at Megans Ln, Redwood City, CA

License number

California A0955131

Issued Date

Apr 2016

Expiration Date

Apr 2018

Professional information

Modified Primer Extension Reactions For Polynucleotide Sequence Detection

US Patent:

6107061, Aug 22, 2000

Filed:

Sep 18, 1999

Appl. No.:

9/399501

Inventors:

Martin D. Johnson - Woodside CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C12P 1934

US Classification:

435 911

Abstract:

The present invention relates to assays for the detection of nucleotide bases at predetermined locations on polynucleotides of interest. The embodiments of the invention include techniques relating to methods in which a primer extension reaction is designed to only extend a single nucleotide base. The invention, in part, relates to the improvement of adding corresponding non-labeled chain terminators to a primer extension reaction so as to increase the accuracy and reliability of nucleotide base determinations made by the assay. The invention is particularly useful when applied to assay methods in which the primer extension reaction is detected by a fluorescent dyes conjugated to chain terminators incorporated in the extension reactions. A first embodiment of the invention relates to methods for identifying a nucleotide base at the predetermined location on a polynucleotide of interest. Other embodiments of the invention include compositions for identifying a nucleotide base at a predetermined location on a polynucleotide of interest.