Inventors:
Kieren A. Marr - Baltimore MD, US
Janet F. Staab - Baltimore MD, US
Marta Feldmesser - New York NY, US
Assignee:
YESHIVA UNIVERSITY - New York NY
THE JOHNS HOPKINS UNIVERSITY - Baltimore MD
International Classification:
G01N 33/569, C07K 16/12, C07K 16/14, G01N 33/577, C12M 1/34
US Classification:
435 792, 435 71, 4352872, 5303875
Abstract:
Fungal infections are difficult to diagnose. The most common filamentous fungal infection, aspergillosis, carries with it a high mortality. Culture of the organism is difficult and obtaining samples, e.g., though a lung biopsy, sometimes causes morbidity. Biomarkers that indicate ‘early’ infection in it development are sought after. One such biomarker is detection of galactomannan (GM), a polysaccharide that is attached to hyphal cell walls and secreted during growth of the organism. Galactomannan is excreted in urine. Disclosed herein is a lateral flow assay comprising monoclonal antibodies that recognize specific residues of for detecting GM in urine samples to provide a point-of-care detection device to allow for frequent screening and early diagnosis in patients at high risk for infection.