MARK T GROUDINE
Radiology at Pacific St, Seattle, WA

5885797, Mar 23, 1999

Aug 27, 1996

8/704931

C. M. Amy Chen - Brookline MA
Norbert Kraut - Seattle WA
Mark Groudine - Seattle WA
Harold Weintraub - late of Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

C12P 2106

435 691

A novel gene, Inhibitor of MyoD Family (I-mf), is provided which encodes novel proteins, I-mfa, I-mfb and I-mfc, involved in regulation of myogenesis during vertebrate development. I-mf is highly expressed in the sclerotome of developing vertebrates and is postulated to play an important role in patterning of the somite and determination sclerotomal cell fate. A unique, C-terminal interactional domain of the I-mf protein mediates physical interactions between I-mfa and members of the MyoD family of transcriptional activators and functions to inhibit transactivation of muscle specific genes by MyoD family members, thereby repressing myogenesis. Further characterization of I-mf activity shows that I-mf associates with MyoD family member proteins and retains them in the cytoplasm by masking their nuclear localization signals. Based on the I-mf genes and proteins disclosed herein, a variety methods and compositions are provided for screening, isolating, and characterizing endogenous and exogenous factors, drugs and therapeutic agents useful to evaluate and/or control myogenesis normal and abnormal development and cell differentiation.

5866338, Feb 2, 1999

Jun 6, 1997

8/870693

Leland H. Hartwell - Seattle WA
Ted A. Weinert - Tucson AR
Sharon E. Plon - Houston TX
Mark T. Groudine - Seattle WA

University of Washington - Seattle WA
Arizona Board of Regents on Behalf of the University of Arizona - Tucson AZ
Fred Hutchinson Cancer Research Center - Seattle WA

C12Q 168

435 6

Human checkpoint huCDC34, huRAD9. sub. compA, and huRAD9. sub. compB cDNAs shown in FIGS. 1, 2, and 3. A method for isolating a human checkpoint cDNA that is capable of restoring growth at a restrictive temperature in a yeast test cell, wherein the yeast test cell comprises a genome having a first gene that forms a DNA strand break at a restrictive temperature and a second gene that fails to induce a cell cycle arrest in response to the DNA strand break, whereby the growth of the yeast test cell is inhibited at the restrictive temperature, the method comprising the steps of: obtaining a human cDNA library comprising a plurality of human cDNA clones; inserting the human cDNA clones individually into plasmid vectors comprising a selectable marker gene; transforming a culture of the yeast test cells with the plasmid vectors from the preceding step; selecting for yeast test cells transformed with the selectable marker gene; growing the selected transformants at the restrictive temperature and isolating a candidate transformant capable of growing at the restrictive temperature; and identifying the human cDNA carried by the candidate transformant as a human checkpoint cDNA by sequencing the human cDNA carried by the candidate transformant and determining that the human cDNA is less than 50% homologous with both the first gene and the second gene. Also yeast checkpoint RAD17, RAD24, MEC1, MEC2, and MEC3 cDNAs shown in FIGS. 4-8.

6074625, Jun 13, 2000

Feb 28, 1997

8/808203

M. Frederick Hawthorne - Encino CA
Mark T. Groudine - Seattle WA

Neutron Therapies Inc. - Seattle WA

A61K 5100, A61M 3614

424 111

Boron containing compounds are targeted to hormonally responsive cells by contacting the cells with a conjugate of a boron containing moiety conjugated to a ligand having binding specificity for an intracellular receptor of the cell. The conjugates are useful for imaging or killing hormonally responsive cells, such as hormonally responsive tumor cells. For target cell killing, the cells are contacted with a. sup. 10 B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells. The. sup. 10 B containing moiety becomes associated with the hormone receptor of the cells, which may then be irradiated with neutrons to kill the cells by boron neutron capture. For target cell imaging, the cells are contacted with a. sup. 11 B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells, and the boron that becomes associated with the hormone receptor of the cells may then be imaged using magnetic resonance imaging techniques.

5674996, Oct 7, 1997

Feb 18, 1994

8/198446

Leland H. Hartwell - Seattle WA
Ted A. Weinert - Tucson AZ
Sharon E. Plon - Houston TX
Mark T. Groudine - Seattle WA

University of Washington - Seattle WA
Arizona Board of Regents on behalf of the University of Arizona - Tucson AZ
Fred Hutchinson Cancer Research Center - Seattle WA

C07H 2104

536 2431

Human checkpoint huCDC34, huRAD9. sub. compA, and huRAD9. sub. compB cDNAs shown in SEQ ID Nos:7-9. A method for isolating a human checkpoint cDNA that is capable of restoring growth at a restrictive temperature in a yeast test cell, wherein the yeast test cell comprises a genome having a first gene that forms a DNA strand break at a restrictive temperature and a second gene that fails to induce a cell cycle arrest in response to the DNA strand break, whereby the growth of the yeast test cell is inhibited at the restrictive temperature, the method comprising the steps of: obtaining a human cDNA library comprising a plurality of human cDNA clones; inserting the human cDNA clones individually into plasmid vectors comprising a selectable marker gene; transforming a culture of the yeast test cells with the plasmid vectors from the preceding step; selecting for yeast test cells transformed with the selectable marker gene; growing the selected transformants at the restrictive temperature and isolating a candidate transformant capable of growing at the restrictive temperature; and identifying the human cDNA carried by the candidate transformant as a human checkpoint cDNA by sequencing the human cDNA carried by the candidate transformant and determining that the human cDNA is less than 50% homologous with both the first gene and the second gene. Also yeast checkpoint RAD17, RAD24, MEC1, MEC2, and MEC3 cDNAs shown in SEQ ID Nos:10-19.

5543319, Aug 6, 1996

Mar 31, 1995

8/415354

R. E. Keith Fournier - Mercer Island WA
Mark T. Groudine - Seattle WA
Ellen S. Dieken - Issaquah WA
Elliot M. Epner - Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

C12N 512, C12N 526, C12N 1508

43524026

An avian/mammalian microcell hybrid immortalized pre B cell line containing a mammalian chromosome that carries a selectable marker has been produced. The avian/mammalian microcell hybrid immortalized pre B cell line can be used for introducing predetermined point mutations into specific mammalian chromosomal loci via high frequency homologous recombination.