MARK J GOLDSMITH
Broker in Boston, MA
License number
Massachusetts 9031116
Issued Date
Jun 11, 1999
Expiration Date
Mar 13, 2001
Type
Salesperson
Address
Address
Boston, MA 02134
Professional information
Methods And Polynucleotide Constructs For Treating Host Cells For Infection Or Hyperproliferative Disorders
US Patent:
5861290, Jan 19, 1999
Filed:
Oct 22, 1992
Appl. No.:
7/965039
Inventors:
Mark A. Goldsmith - West Roxbury MA
Robert O. Ralston - San Francisco CA
Robert O. Ralston - San Francisco CA
International Classification:
C12N 1509, C12N 1511, C12N 1563, C12N 1586
US Classification:
4351723
Abstract:
Host cells may be treated for an infection or a hyperproliferative disorder which is characterized by the presence, in the affected cells, of a trans-acting factor capable of regulating gene expression by inserting into the cells a polynucleotide construct having a cis-acting regulatory sequence which is regulated by the trans-acting factor and an effector gene which renders said cell susceptible to protection or destruction. For example, the cis-acting region may be homologous to the HIV tar region, and the effector gene may encode ricin A or HSV-1 thymidine kinase. Upon infection with HIV, the HIV tat protein activates the tar region, and induces transcription and expression of ricin A, resulting in cell death, or of HSV-1 tk, resulting in cell death upon treatment with dideoxynucleoside agents such as acyclovir and gancyclovir.