DR. MARK DEVOY REISBIG, M.D., PH.D.
Anesthesiologist Assistant at Nebraska Medical Ctr, Omaha, NE
License number
Nebraska TEP6088
Category
Osteopathic Medicine
Type
Anesthesiology
Address
Address
984455 Nebraska Medical Ctr, Omaha, NE 68198
Phone
(402) 559-4081
(402) 559-7372 (Fax)
(402) 559-7372 (Fax)
Personal information
See more information about MARK DEVOY REISBIG at radaris.comName
Address
Phone
13823 Arbor St, Omaha, NE 68144
5016 California St, Omaha, NE 68132
6712 162Nd St, Omaha, NE 68135
Professional information
Dr. Mark D Reisbig, Omaha NE - MD (Doctor of Medicine)
Specialties:
Anesthesiology
Address:
984455 Nebraska Medical Ctr, Omaha 68198
(402) 559-4081 (Phone), (402) 559-7372 (Fax)
985524 Nebraska Medical Ctr, Omaha 68198
601 N 30Th St SUITE 1609, Omaha 68131
CREIGHTON UNIVERSITY MEDICAL CENTER
601 N 30Th St SUITE 1609, Omaha 68131
(402) 449-4590 (Phone), (402) 449-4982 (Fax)
(402) 559-4081 (Phone), (402) 559-7372 (Fax)
985524 Nebraska Medical Ctr, Omaha 68198
601 N 30Th St SUITE 1609, Omaha 68131
CREIGHTON UNIVERSITY MEDICAL CENTER
601 N 30Th St SUITE 1609, Omaha 68131
(402) 449-4590 (Phone), (402) 449-4982 (Fax)
Languages:
English
Hospitals:
984455 Nebraska Medical Ctr, Omaha 68198
985524 Nebraska Medical Ctr, Omaha 68198
601 N 30Th St SUITE 1609, Omaha 68131
CREIGHTON UNIVERSITY MEDICAL CENTER
601 N 30Th St SUITE 1609, Omaha 68131
Alegent Creighton Health Creighton University Medical Center
601 North 30Th St, Omaha 68131
985524 Nebraska Medical Ctr, Omaha 68198
601 N 30Th St SUITE 1609, Omaha 68131
CREIGHTON UNIVERSITY MEDICAL CENTER
601 N 30Th St SUITE 1609, Omaha 68131
Alegent Creighton Health Creighton University Medical Center
601 North 30Th St, Omaha 68131
Education:
Medical School
Creighton University
Graduated: 2008
Creighton University
Graduated: 2008
Mark Devoy Reisbig, Omaha NE
Specialties:
Anesthesiologist
Address:
Unmc Anesthesiology, Omaha, NE 68198
Rapid Nucleic Acid Isolation Method And Compositions
US Patent:
2007007, Apr 5, 2007
Filed:
Jul 21, 2004
Appl. No.:
10/565358
Inventors:
Ashfaque Hossain - Omaha NE, US
Nancy Hanson - Gretna NE, US
Mark Reisbig - Omaha NE, US
Nancy Hanson - Gretna NE, US
Mark Reisbig - Omaha NE, US
International Classification:
C12Q 1/68, C07H 21/02
US Classification:
435006000, 536025400
Abstract:
A method of isolating RNA from a biological specimen is provided, whereby a biological specimen is contacted with an admixture of (i) a mono-phasic solution of phenol and guanidine isothiocyanate and (ii) a lysis buffer under conditions and for a time appropriate to form a homogenate. Next, the homogenate is admixed with a water-immiscible organic solvent under conditions and for a time appropriate to form an aqueous phase and an organic phase. The aqueous phase is then contacted with a C-Clower alcohol under conditions and for a time to form a precipitated RNA. The precipitated RNA is then recovered by centrifugation and decanting of the aqueous phase. The method can also be used to isolate total RNA. In an alternative embodiment, the biological sample is contacted with (i) a lysis buffer, and (ii) a mono-phasic solution of phenol and guanidine isothiocyanate under conditions and for a time appropriate to form a homogenate. The remaining steps of this embodiment are the same as above.