Inventors:
David A. Schulsinger - New York NY
Philip S. Li - Flushing NY
Marc Goldstein - New York NY
Assignee:
Cornell Research Foundation, Inc. - Ithaca NY
International Classification:
A01N 102
Abstract:
A method for autotransplantation, is provided including the steps of (a) obtaining a piece of tissue from one part of a body, (b) preserving the tissue by contacting it with a tissue preserving medium, without tissue culturing occurring, and (c) grafting the tissue in another part of the body. The method is especially advantageous for the repair of hypospadias using buccal mucosa tissue. The tissue preserving medium preferably is a solution of (a) 80-120 mM NaCl, (b) 3-6 mM KCl, (c) 0. 1-0. 3 mM MgSO. sub. 4. 7H. sub. 2 O, (d) 1-3 mM CaCl. sub. 2. 2H. sub. 2 O, (e) 0. 2-0. 6 mM NaH. sub. 2 PO. sub. 4, (f) 1. 5-4 mM glucose, (g) 15-25 mM Na lactate, (h) 0. 2-0. 6 mM Na pyruvate, (i) 0. 01-0. 05 mM phenol red, (j) 0. 1-0. 4 mM L-glutamine, (k) 2-35 mM sodium bicarbonate, (l) 125-200 units/ml penicillin-G, (m) 40-60. mu.