Inventors:
George M. WHITESIDES - Newton MA, US
Scott T. PHILLIPS - Cambridge MA, US
Andres W. MARTINEZ - Cambridge MA, US
Manish J. BUTTE - Boston MA, US
Amy WONG - Saratoga CA, US
Samuel W. THOMAS - Boston MA, US
Hayat SINDI - Cambridge, GB
Sarah J. VELLA - Ontario, CA
Emanuel CARRILHO - Chestnut Hill MA, US
Katherine A. MIRICA - Cambridge MA, US
Yanyan LIU - Cambridge MA, US
Assignee:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE - Cambridge MA
International Classification:
G01N 21/00, G01N 31/22, B01L 3/00, G03F 7/20, B29C 59/00, B05D 1/28
US Classification:
436164, 422 56, 422 99, 430325, 264241
Abstract:
Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.