DR. LUIS HORACIO TOLEDO, MD
Radiology at Oakland Dr, Kalamazoo, MI

2003015, Aug 21, 2003

Jun 26, 2002

10/186158

Vladimir Serebrennikov - Krasnoyarsk, RU
David Lucas - Lafayetta CA, US
Luis Toledo - Portage MI, US

A61K047/00

604/004010, 435/002000

Methods of cooling blood platelet suspensions which can be stored and preserved for extended periods of time. The normal morphology of platelets and their ability to function are substantially maintained. The steps include preparing a platelet suspension having blood platelets, a carbohydrate and at least one biocompatible polymer to assist in stabilizing platelet membranes. The platelet suspension may be cooled to a temperature of less than approximately 10 degrees C. at a rate of cooling greater than 1 degree C./min. The platelet suspension may be kept at a storage temperature ranging from approximately -1 to 6 degrees C. Additionally, methods are provided for maintaining the biological activity of blood platelets. Platelet suspensions may be initially prepared which include platelets, sucrose, verapamil, magnesium chloride and a biocompatible polymer. Cooling of the platelet suspension may be followed at a cooling rate ranging from approximately 1 to 12 degrees C./min or faster to a temperature below 10 degrees C. The cooled platelet suspension may be thus stored at a storage temperatures as high as 6 degrees C.

2007000, Jan 11, 2007

Jul 11, 2006

11/456816

Luis Toledo - Portage MI, US
Fernando Lopez - Vicksburg MI, US

A01N 1/02

435001100

The present invention provides methods of preserving, storing and transplanting mammalian donor organs. The method includes the cooling of refrigeration preservation, loading pre-freezer preservation, cryopreservation, and washing solutions at least containing polyvinylpyrrolidone, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, and a steroid to a temperature of 2° to 4° C. and/or of 0° to 2° C., harvesting a donor organ, perfusing it with one or more of the solution, immersing it in one or more of the solutions and storing it at a temperature above 0° C. or at a temperatures below 0° C. including −20° C., −80° C. and −196° C. The cryopreservation solution also contains cryopreservative agents. Preserved organs may be transplanted directly from refrigeration storage or from freezer storage by cooling the washing refrigeration preservation solutions to 2° to 4° C., perfusing the organ with washing solution and then preservation solution, and transplanting it.

2005011, May 26, 2005

Nov 21, 2003

10/719621

Luis Toledo - Portage MI, US
Edward Hessler - Kalamazoo MI, US
Harold Karnes - Portage MI, US

C07D413/14, C07D043/14

540601000, 544295000, 544114000

The invention includes piperazinyl pyrimidinyl nitroso compounds of the formula (II) and N-Nitrosometoprolol and pharmaceutically acceptable salts thereof which are useful in treating one or more of the following ischemic diseases, coronary heart disease, stroke, hemorrhagic shock, peripheral vascular disease (of both the upper and lower extremities), abdominal vascular insufficiency and transplant related surgery.