LUIS C CANO
Electrician at West Rd, Houston, TX
License number
Texas 382157
Expiration Date
Oct 10, 2017
Category
Apprentice Electrician
Address
Address
411 West Rd WEST APT 108, Houston, TX 77038
Phone
(832) 727-8843
Professional information
President At Jl Marketing
Position:
President at JL MARKETING
Location:
Houston, Texas Area
Industry:
Marketing and Advertising
Work:
JL MARKETING
-
President
Introduction Of Structural Affinity Handles As A Tool In Selective Nucleic Acid Separations
US Patent:
2006016, Jul 20, 2006
Filed:
Dec 16, 2003
Appl. No.:
10/737403
Inventors:
Richard Willson - Houston TX, US
Luis Cano - Houston TX, US
Luis Cano - Houston TX, US
International Classification:
C12Q 1/68, C07H 21/04, C12P 19/34
US Classification:
435006000, 435091200, 536025400
Abstract:
The method is used for separating nucleic acids and other similar constructs. It involves selective introduction, enhancement, or stabilization of affinity handles such as single-strandedness in the undesired (or desired) nucleic acids as compared to the usual structure (e.g., double-strandedness) of the desired (or undesired) nucleic acids. The undesired (or desired) nucleic acids are separated from the desired (or undesired) nucleic acids due to capture by methods including but not limited to immobilized metal affinity chromatography, immobilized single-stranded DNA binding (SSB) protein, and immobilized oligonucleotides. The invention is useful to: remove contaminating genomic DNA from plasmid DNA; remove genomic DNA from plasmids, BACs, and similar constructs; selectively separate oligonucleotides and similar DNA fragments from their partner strands; purification of aptamers, (deoxy)-ribozymes and other highly structured nucleic acids; Separation of restriction fragments without using agarose gels; manufacture recombinant Taq polymerase or similar products that are sensitive to host genomic DNA contamination; and other applications