Inventors:
Carl Wittwer - Salt Lake City UT, US
Andrew Crockett - West Valley UT, US
Brian Caplin - Salt Lake City UT, US
Wade Stevenson - West Jordan UT, US
Lori Wagner - Salt Lake City UT, US
Jian Chen - Sandy UT, US
Noriko Kusukawa - Salt Lake City UT, US
International Classification:
C12Q001/68, C07H021/04
Abstract:
Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.